Franco A M, Momen H, Naiff R D, Moreira C F, Deane M P, Grimaldi Júnior G
Department of Immunology, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil.
Parasitology. 1996 Jul;113 ( Pt 1):39-48. doi: 10.1017/s0031182000066257.
In this study, we have analysed enzyme polymorphism among a group of protozoan parasites of the genus Endotrypanum (Kinetoplastida: Trypanosomatidae). Seventeen stocks of Endotrypanum spp. isolated from sloths (Choloepus didactylus and C. juruanus) in the Amazon Region of Brazil were analysed by enzyme electrophoresis, and their electromorphic profiles were compared with reference strains reported previously. The 16 enzymic loci were analysed, and the strains were classified into zymodemes, each representing parasites with unique enzyme profiles. Each zymodeme was considered as an elementary taxon, and using numerical analyses (cladistic, agglomerative hierarchical and ordination techniques) the genus was shown to be monophyletic and the 12 zymodemes characterized could be divided into 3 groups (A, B, C). The heterogeneous population (which may represent a complex of parasite species or strains variants) showed, however, no correlation with the origin (i.e. host species involved or geographic area of isolation) of Endotrypanum stocks. Eight isolates of Endotrypanum sp. from Rondônia State (Brazil) and a parasite strain from Panama were clustered together into a zymodeme, which was phenetically closely related to the E. monterogeii from Costa Rica. The data indicate that E. schaudinni is a species complex.
在本研究中，我们分析了内锥虫属（动基体目：锥虫科）一组原生动物寄生虫中的酶多态性。通过酶电泳分析了从巴西亚马逊地区的树懒（二趾树懒和侏三趾树懒）分离出的17株内锥虫属菌株，并将它们的电形态图谱与先前报道的参考菌株进行了比较。分析了16个酶位点，并将这些菌株分类为酶型，每个酶型代表具有独特酶谱的寄生虫。每个酶型被视为一个基本分类单元，通过数值分析（分支分析、凝聚层次分析和排序技术）表明该属是单系的，所鉴定的12个酶型可分为3组（A、B、C）。然而，这个异质群体（可能代表寄生虫物种或菌株变体的复合体）与内锥虫菌株的来源（即所涉及的宿主物种或分离的地理区域）没有相关性。来自朗多尼亚州（巴西）的8株内锥虫属分离株和来自巴拿马的一个寄生虫菌株聚为一个酶型，从表型上看，它与来自哥斯达黎加的蒙特罗吉内锥虫密切相关。数据表明，肖丁内锥虫是一个复合种。
