Lebrun A, Lavery R
Laboratoire de Biochimie Théorique, Institut de Biologie Physico-Chimique, Paris, France.
Nucleic Acids Res. 1996 Jun 15;24(12):2260-7. doi: 10.1093/nar/24.12.2260.
Molecular modelling with Jumna is used to study extreme stretching of the DNA double helix. The results, which correlate well with recent nanomanipulation experiments, show how the double helix can be extended to twice its normal length before its base pairs break. Depending on the way the duplex is stretched two types of conformation can occur, either an unwound flat ribbon or a narrow fibre with negatively inclined base pairs. The energetics of both types of deformation are similar and existing structures show that at least the flat ribbon form can exist locally under biological conditions.
使用Jumna进行分子建模来研究DNA双螺旋的极限拉伸。这些结果与最近的纳米操作实验相关性良好，显示了双螺旋在其碱基对断裂之前如何能够扩展到其正常长度的两倍。根据双链拉伸的方式，会出现两种构象类型，要么是展开的扁平带，要么是碱基对呈负倾斜的窄纤维。两种变形类型的能量学相似，现有结构表明至少扁平带形式在生物学条件下可以局部存在。
