Fan H H, Kleven S H, Jackwood M W
Department of Avian Medicine, University of Georgia, Athens 30602-4875, USA.
Avian Dis. 1995 Oct-Dec;39(4):766-77.
Intraspecies genotypic heterogeneity among isolates and strains of M. synoviae (MS), M. iowae (MI), and M. meleagridis (MM) was demonstrated with the arbitrarily primed polymerase chain reaction (AP-PCR) method. The generated banding patterns of amplified DNA indicated a degree of genotypic heterogeneity among MS, MI, and MM isolates and strains tested, whereas isolates from common sources generated clusters with identical banding patterns. The results show that flocks infected with members of the cluster were epidemiologically associated, possibly by a common or point source of infection. This AP-PCR method may be a useful identification tool in epidemiology of mycoplasmosis in poultry.
用任意引物聚合酶链反应（AP-PCR）方法证明了鸡滑液支原体（MS）、衣阿华支原体（MI）和火鸡支原体（MM）的分离株和菌株之间存在种内基因型异质性。扩增DNA产生的条带模式表明，所测试的MS、MI和MM分离株及菌株之间存在一定程度的基因型异质性，而来自共同来源的分离株产生了具有相同条带模式的聚类。结果表明，感染该聚类成员的鸡群在流行病学上存在关联，可能是通过共同或单点感染源。这种AP-PCR方法可能是家禽支原体病流行病学中一种有用的鉴定工具。
