Kartnig T, Göbel I, Heydel B
Institut für Pharmakognosie der Karl-Franzens-Universität Graz, Austria.
Planta Med. 1996 Feb;62(1):51-3. doi: 10.1055/s-2006-957796.
Suspension cultures were established from the shoots of sterile germinated seeds of various provenances of seven Hypericum (H.) species in a half strength modified Murashige and Skoog liquid medium. In most strains of H. perforatum (18 provenances) and all strains of H. maculatum (6 provenances) as well as in the cultures of H. tomentosum, H. bithynicum, H. glandulosum and H. balearicum, hypericin and pseudohypericin could be proven, however, in extremely varying amounts. In general, the pseudohypericin content was significantly higher than that of hypericin. The flavonoid patterns, comprising monomeric quercetin derivatives and dimeric apigenin derivatives, varied among strains over a wide range.
在改良的1/2强度Murashige和Skoog液体培养基中，从7种金丝桃属（H.）植物不同种源的无菌萌发种子的芽中建立了悬浮培养物。在贯叶连翘的大多数菌株（18个种源）、地耳草的所有菌株（6个种源）以及绒毛金丝桃、比提尼亚金丝桃、腺金丝桃和巴利阿里金丝桃的培养物中，均可检测到金丝桃素和假金丝桃素，但其含量差异极大。一般来说，假金丝桃素的含量明显高于金丝桃素。由单体槲皮素衍生物和二聚芹菜素衍生物组成的黄酮类化合物模式在不同菌株间差异很大。
