Iriyama K, Tsuchibashi T, Miki C, Kalembeyi I, Li H, Urata H, Saitoh R
Second Department of Surgery, Mie University School of Medicine, Tsu, Japan.
Nutrition. 1996 Feb;12(2):79-82. doi: 10.1016/0899-9007(96)90703-0.
The elimination rate of emulsion triglyceride (TG) from plasma was investigated in Japanese subjects by a plasma TG clamp technique. Two different studies were performed. In Study 1, a lipid emulsion (20% long-chain triglyceride emulsion: LCT) was infused into a healthy research associate to achieve a certain concentration of TG in the plasma. Thereafter, the infusion rate was adjusted to maintain the chosen concentrations of TG in plasma (namely, 4-5 mmol/L, 3-4 mmol/L, and approximately 2 mmol/L) over a period of 160 min by measuring plasma TG concentrations at 10-min intervals. Concentrations of TG in plasma were clamped within 2.13 +/- 0.13 mmol/L by an infusion rate of 0.10 g.kg-1.h-1, within 3.34 +/- 0.20 mmol/L by an infusion rate of 0.14 g.kg-1.h-1, and within 4.46 +/- 0.22 mmol/L by an infusion rate of 0.11 g.kg-1.h-1. The mean rate of infusion of emulsified TG that had maintained the steady concentrations of TG in plasma was limited to the very narrow range of 0.12 +/- 0.02 g of TG.kg-1.h-1 regardless of the chosen concentration of TG in plasma. Concentrations of nonesterified fatty acids (NEFA) also remained at a fixed level of 1.378 +/- 0.103 mEq/L regardless of the chosen concentration of TG in the plasma. Study 2 was undertaken to determine whether plasma TG concentration reached a plateau during a period when emulsion TG was infused into three different subjects at a rate of 0.12 g.kg-1.h-1. The plasma TG concentrations were steady at a level of 2.04 +/- 0.32 mmol/L, and the plasma NEFA concentrations remained at a fixed level of 1.33 +/- 0.13 mEq/L, over a period of 160 min after 50-min priming infusion. These results indicate that the plasma TG elimination rate was limited to the narrow range of 0.12 +/- 0.02 g.kg-1.h-1 when the fat emulsion was infused into Japanese subjects in a steady state. However, the plasma TG elimination rate in Japanese subjects appeared to be lower than that of Europeans. This may be due to a difference in lipoprotein lipase activity caused by different dietary habits, namely, a lower fat intake.
采用血浆甘油三酯钳夹技术，在日本受试者中研究了血浆中乳剂甘油三酯（TG）的清除率。进行了两项不同的研究。在研究1中，将脂质乳剂（20%长链甘油三酯乳剂：LCT）输注到一名健康的研究助理体内，以使血浆中TG达到一定浓度。此后，通过每隔10分钟测量血浆TG浓度，调整输注速率，在160分钟内维持血浆中选定的TG浓度（即4 - 5 mmol/L、3 - 4 mmol/L和约2 mmol/L）。通过0.10 g·kg⁻¹·h⁻¹的输注速率，血浆TG浓度被钳制在2.13±0.13 mmol/L以内；通过0.14 g·kg⁻¹·h⁻¹的输注速率，血浆TG浓度被钳制在3.34±0.20 mmol/L以内；通过0.11 g·kg⁻¹·h⁻¹的输注速率，血浆TG浓度被钳制在4.46±0.22 mmol/L以内。维持血浆中TG稳定浓度的乳化TG的平均输注速率被限制在非常窄的范围内，即0.12±0.02 g TG·kg⁻¹·h⁻¹，无论血浆中选定的TG浓度如何。无论血浆中选定的TG浓度如何，非酯化脂肪酸（NEFA）的浓度也保持在1.378±0.103 mEq/L的固定水平。进行研究2以确定在以0.12 g·kg⁻¹·h⁻¹的速率向三名不同受试者输注乳剂TG的期间，血浆TG浓度是否达到平台期。在50分钟的起始输注后的160分钟内，血浆TG浓度稳定在2.04±0.32 mmol/L水平，血浆NEFA浓度保持在1.33±0.13 mEq/L的固定水平。这些结果表明，当脂肪乳剂以稳定状态输注到日本受试者体内时，血浆TG清除率被限制在0.12±0.02 g·kg⁻¹·h⁻¹的窄范围内。然而，日本受试者的血浆TG清除率似乎低于欧洲人。这可能是由于不同饮食习惯导致的脂蛋白脂肪酶活性差异，即较低的脂肪摄入量。
