通过甘油三酯钳夹技术测定日本受试者血浆中脂肪乳剂颗粒的清除率。

Elimination rate of fat emulsion particles from plasma in Japanese subjects as determined by a triglyceride clamp technique.

作者信息

Iriyama K, Tsuchibashi T, Miki C, Kalembeyi I, Li H, Urata H, Saitoh R

机构信息

Second Department of Surgery, Mie University School of Medicine, Tsu, Japan.

出版信息

Nutrition. 1996 Feb;12(2):79-82. doi: 10.1016/0899-9007(96)90703-0.

DOI:10.1016/0899-9007(96)90703-0

PMID:8724376

Abstract

The elimination rate of emulsion triglyceride (TG) from plasma was investigated in Japanese subjects by a plasma TG clamp technique. Two different studies were performed. In Study 1, a lipid emulsion (20% long-chain triglyceride emulsion: LCT) was infused into a healthy research associate to achieve a certain concentration of TG in the plasma. Thereafter, the infusion rate was adjusted to maintain the chosen concentrations of TG in plasma (namely, 4-5 mmol/L, 3-4 mmol/L, and approximately 2 mmol/L) over a period of 160 min by measuring plasma TG concentrations at 10-min intervals. Concentrations of TG in plasma were clamped within 2.13 +/- 0.13 mmol/L by an infusion rate of 0.10 g.kg-1.h-1, within 3.34 +/- 0.20 mmol/L by an infusion rate of 0.14 g.kg-1.h-1, and within 4.46 +/- 0.22 mmol/L by an infusion rate of 0.11 g.kg-1.h-1. The mean rate of infusion of emulsified TG that had maintained the steady concentrations of TG in plasma was limited to the very narrow range of 0.12 +/- 0.02 g of TG.kg-1.h-1 regardless of the chosen concentration of TG in plasma. Concentrations of nonesterified fatty acids (NEFA) also remained at a fixed level of 1.378 +/- 0.103 mEq/L regardless of the chosen concentration of TG in the plasma. Study 2 was undertaken to determine whether plasma TG concentration reached a plateau during a period when emulsion TG was infused into three different subjects at a rate of 0.12 g.kg-1.h-1. The plasma TG concentrations were steady at a level of 2.04 +/- 0.32 mmol/L, and the plasma NEFA concentrations remained at a fixed level of 1.33 +/- 0.13 mEq/L, over a period of 160 min after 50-min priming infusion. These results indicate that the plasma TG elimination rate was limited to the narrow range of 0.12 +/- 0.02 g.kg-1.h-1 when the fat emulsion was infused into Japanese subjects in a steady state. However, the plasma TG elimination rate in Japanese subjects appeared to be lower than that of Europeans. This may be due to a difference in lipoprotein lipase activity caused by different dietary habits, namely, a lower fat intake.

摘要

采用血浆甘油三酯钳夹技术，在日本受试者中研究了血浆中乳剂甘油三酯（TG）的清除率。进行了两项不同的研究。在研究1中，将脂质乳剂（20%长链甘油三酯乳剂：LCT）输注到一名健康的研究助理体内，以使血浆中TG达到一定浓度。此后，通过每隔10分钟测量血浆TG浓度，调整输注速率，在160分钟内维持血浆中选定的TG浓度（即4 - 5 mmol/L、3 - 4 mmol/L和约2 mmol/L）。通过0.10 g·kg⁻¹·h⁻¹的输注速率，血浆TG浓度被钳制在2.13±0.13 mmol/L以内；通过0.14 g·kg⁻¹·h⁻¹的输注速率，血浆TG浓度被钳制在3.34±0.20 mmol/L以内；通过0.11 g·kg⁻¹·h⁻¹的输注速率，血浆TG浓度被钳制在4.46±0.22 mmol/L以内。维持血浆中TG稳定浓度的乳化TG的平均输注速率被限制在非常窄的范围内，即0.12±0.02 g TG·kg⁻¹·h⁻¹，无论血浆中选定的TG浓度如何。无论血浆中选定的TG浓度如何，非酯化脂肪酸（NEFA）的浓度也保持在1.378±0.103 mEq/L的固定水平。进行研究2以确定在以0.12 g·kg⁻¹·h⁻¹的速率向三名不同受试者输注乳剂TG的期间，血浆TG浓度是否达到平台期。在50分钟的起始输注后的160分钟内，血浆TG浓度稳定在2.04±0.32 mmol/L水平，血浆NEFA浓度保持在1.33±0.13 mEq/L的固定水平。这些结果表明，当脂肪乳剂以稳定状态输注到日本受试者体内时，血浆TG清除率被限制在0.12±0.02 g·kg⁻¹·h⁻¹的窄范围内。然而，日本受试者的血浆TG清除率似乎低于欧洲人。这可能是由于不同饮食习惯导致的脂蛋白脂肪酶活性差异，即较低的脂肪摄入量。