Gago-Martinez A, Rodriguez-Vazquez J A, Thibault P, Quilliam M A
Department of Analytical Chemistry and Nutrition, University of Vigo, Spain.
Nat Toxins. 1996;4(2):72-9. doi: 10.1002/19960402nt3.
Mussel aquaculture is an important industry for the Galician Rias, located in northwestern Atlantic coast of Spain. Since 1976 this region has been seriously affected by incidents of paralytic and diarrhetic shellfish poisoning (PSP and DSP). A particularly bad episode occurred in 1993, when the toxic event lasted for an unusually long period. Many people were stricken ill with unusual symptoms. In this paper we report on the chemical analysis of toxic 1993 mussel samples, using the techniques of liquid chromatography and capillary electrophoresis coupled with mass spectrometry. These analyses revealed a very complex toxin profile, with both PSP and DSP toxins present. Two DSP toxins, okadaic acid and DTX2, were observed, while the primary PSP toxins were B1 and the decarbamoylated derivatives of saxitoxin, GTX2 and GTX3. Small amounts of saxitoxin and other as yet unidentified PSP toxins were observed.
贻贝养殖是位于西班牙西北大西洋海岸的加利西亚里亚斯地区的一项重要产业。自1976年以来，该地区一直受到麻痹性和腹泻性贝类中毒事件（PSP和DSP）的严重影响。1993年发生了一次特别严重的事件，当时毒性事件持续了异常长的时间。许多人出现了异常症状。在本文中，我们报告了使用液相色谱和毛细管电泳与质谱联用技术对1993年有毒贻贝样本进行的化学分析。这些分析揭示了一个非常复杂的毒素谱，同时存在PSP和DSP毒素。观察到两种DSP毒素，即冈田酸和DTX2，而主要的PSP毒素是B1以及石房蛤毒素的脱氨甲酰化衍生物GTX2和GTX3。还观察到少量的石房蛤毒素和其他尚未鉴定的PSP毒素。
