Padrón D, Lindley V A, Pfeiler E
Departmento de Ciencias Marinas, Instituto Technológico y de Estudios Superiores de Monterrey (ITESM), Guaymas, Sonora, Mexico.
Lipids. 1996 May;31(5):513-9. doi: 10.1007/BF02522645.
During metamorphosis of bonefish (Albula sp.) larvae (leptocephali) all energy requirements are provided by breakdown of endogenous compounds, with lipid catabolism accounting for about 80% of total energy production. The principal objective of the present study was to characterize the lipid classes and fatty acids utilized. Analysis of whole-body lipid content indicated that larvae lost about half (3.6 mg) of their total lipid during the 10-d period. Percentages of neutral and polar lipid in early metamorphosing larvae were 64.2 and 35.8%, respectively; these values showed little change during metamorphosis, indicating that both lipid classes were catabolized. Triacylglycerols, the principal neutral lipid of all metamorphic stages, decreased by 1.8 mg, accounting for half of the decrease in total lipid. Levels of phosphatidylethanolamine, the principal polar lipid in early larvae, decreased by more than 50% during metamorphosis; levels of phosphatidylcholine, which was not detected in early larvae, increased. Fatty acids showing the largest net decreases, presumedly used as energy sources, were 16:0 (30.4%), 14:0 (13.8%), 16:1n-7 (12.2%), 20:5n-3 (7.7%), 18:1n-9 (7.4%), and 18:4n-3 (6.9%). Most of 22:6n-3, the second most abundant fatty acid of early larvae, was conserved.
在海鲢（Albula sp.）幼体（柳叶状幼体）变态期间，所有能量需求均由内源性化合物的分解提供，其中脂质分解代谢约占总能量产生的80%。本研究的主要目的是对所利用的脂质类别和脂肪酸进行表征。全身脂质含量分析表明，幼体在10天的时间内损失了约一半（3.6毫克）的总脂质。早期变态幼体中中性脂质和极性脂质的百分比分别为64.2%和35.8%；这些值在变态过程中变化不大，表明这两类脂质都被分解代谢。三酰甘油是所有变态阶段的主要中性脂质，减少了1.8毫克，占总脂质减少量的一半。早期幼体中的主要极性脂质磷脂酰乙醇胺的水平在变态期间下降了50%以上；早期幼体中未检测到的磷脂酰胆碱的水平则有所增加。净减少量最大的脂肪酸，推测用作能量来源，分别为16:0（30.4%）、14:0（13.8%）、16:1n-7（12.2%）、20:5n-3（7.7%）、18:1n-9（7.4%）和18:4n-3（6.9%）。早期幼体中第二丰富的脂肪酸22:6n-3的大部分得以保留。
