Black W C, Vanlandingham D L, Sweeney W P, Wasieloski L P, Calisher C H, Beaty B J
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
J Clin Microbiol. 1995 Dec;33(12):3179-82. doi: 10.1128/jcm.33.12.3179-3182.1995.
Single-strand conformation polymorphism analysis was developed to differentiate the small RNA segments of three California serogroup bunyaviruses. The small RNA segments of La Crosse, snowshoe hare, and Tahyna viruses were reverse transcribed and PCR amplified. The cDNAs were then denatured, rapidly chilled to promote intrastrand reassociation, separated electrophoretically on a nondenaturing gel at room temperature, and silver stained. The resulting single-strand conformation polymorphism patterns were specific for the respective viruses. This molecular technique offers great potential for virus typing and taxonomic studies.
单链构象多态性分析技术被用于区分三种加利福尼亚血清群布尼亚病毒的小RNA片段。对拉克罗斯病毒、雪兔病毒和塔希纳病毒的小RNA片段进行逆转录和PCR扩增。然后将cDNA变性，迅速冷却以促进链内重缔合，在室温下于非变性凝胶上进行电泳分离，并用银染色。所得的单链构象多态性模式对各自的病毒具有特异性。这种分子技术在病毒分型和分类学研究方面具有巨大潜力。
