Cauchi M R, Doultree J C, Marshall J A, Wright P J
Department of Microbiology, Monash University, Clayton, Victoria, Australia.
J Med Virol. 1996 May;49(1):70-6. doi: 10.1002/(SICI)1096-9071(199605)49:1<70::AID-JMV12>3.0.CO;2-6.
Five small round-structured viruses (SRSVs) associated with gastroenteritis in Victoria, Australia, from January to November 1994 were examined by sequencing cDNA prepared from faecal samples using RT-PCR. The sequence of the 3' half (3.8 kb) of the genome of one of these viruses, Camberwell, was determined. Camberwell virus was related most closely to Bristol and Lordsdale viruses, and belonged to the genetic group of SRSVs containing Bristol, Lordsdale, Toronto, OTH-25, Mexico, and Hawaii viruses. The amino acid identities between Camberwell and Bristol viruses for proteins encoded by ORF1 (partial), ORF2, and ORF3 were 99%, 98%, and 90%, respectively. A highly variable region in ORF3 corresponding to amino acid residues 123 to 169 (Bristol and Camberwell numbering) were identified. Short segments of ORF1 (polymerase region) and the highly variable ORF3 region was analysed for the other four viruses. The results obtained indicated the potential usefulness of the variable region in distinguishing between closely related viruses.
1994年1月至11月期间，从澳大利亚维多利亚州收集了5份与肠胃炎相关的小圆结构病毒（SRSV）粪便样本，采用逆转录聚合酶链反应（RT-PCR）对样本中的病毒cDNA进行测序分析。确定了其中一株病毒坎伯韦尔病毒（Camberwell）基因组3'端一半（3.8 kb）的序列。坎伯韦尔病毒与布里斯托尔病毒（Bristol）和洛兹代尔病毒（Lordsdale）亲缘关系最近，属于包含布里斯托尔、洛兹代尔、多伦多、OTH-25、墨西哥和夏威夷病毒的SRSV遗传组。坎伯韦尔病毒与布里斯托尔病毒在开放阅读框1（部分）、开放阅读框2和开放阅读框3编码的蛋白质之间的氨基酸同一性分别为99%、98%和90%。在开放阅读框3中鉴定出一个高度可变区域，对应于氨基酸残基123至169（布里斯托尔和坎伯韦尔编号）。对其他四种病毒的开放阅读框1（聚合酶区域）短片段和高度可变的开放阅读框3区域进行了分析。所得结果表明该可变区域在区分密切相关病毒方面具有潜在用途。
