从西班牙腹泻羔羊中分离出的大肠杆菌菌株的血清群、毒素及抗生素耐药性

Serogroups, toxins and antibiotic resistance of Escherichia coli strains isolated from diarrhoeic lambs in Spain.

作者信息

Blanco J, Cid D, Blanco J E, Blanco M, Ruiz Santa Quiteira J A, de la Fuente R

机构信息

Departmento de Microbiología y Parasitología, Facultad de Veterinaria, Universidad de Santiago, Lugo, Spain.

出版信息

Vet Microbiol. 1996 Apr;49(3-4):209-17. doi: 10.1016/0378-1135(95)00188-3.

DOI:10.1016/0378-1135(95)00188-3

PMID:8734638

Abstract

One hundred and forty-four Escherichia coli strains isolated from 144 diarrhoeic lambs (5 to 21 days old) from 38 flocks in Spain were serotyped and investigated for production of enterotoxins (LT and STa), verotoxins (VT1 and VT2), cytotoxic necrotizing factors (CNF1 and CNF2), alpha-haemolysin (Hly) and enterohaemolysin (EntHly), for necrotic and lethal activities and for antibiotic resistance. The strains belonged to 39 different serogroups; however, 58% were of one of 13 serogroups (O4, O6, O7, O8, O9, O11, O23, O26, O77, O80, O101, O103 and O161) and only four of them (O8, O9, O11 and O77) accounted for 34% of strains. In total 10 (7%) toxigenic strains were detected: two LT+, two VT1+ EntHly+, four VT1+ EntHly-, one CNF1+ Hly+ and one CNF2+. The highest percentages of antibiotic resistance were reached in the group of antibiotics (tetracycline, streptomycin, sulphadiazine, ampicillin, kanamycin, neomycin, chloramphenicol, trimethoprim and cotrimoxazole) that are most generally used by Spanish veterinary clinics. We conclude that E. coli strains isolated from diarrhoeic lambs are not generally toxigenic and belong to a large number of serogroups.

摘要

从西班牙38个羊群中144只腹泻羔羊（5至21日龄）分离出144株大肠杆菌，对其进行血清分型，并检测其肠毒素（LT和STa）、志贺毒素（VT1和VT2）、细胞毒性坏死因子（CNF1和CNF2）、α-溶血素（Hly）和肠溶血素（EntHly）的产生情况、坏死和致死活性以及抗生素耐药性。这些菌株属于39个不同的血清群；然而，58%属于13个血清群之一（O4、O6、O7、O8、O9、O11、O23、O26、O77、O80、O101、O103和O161），其中只有4个（O8、O9、O11和O77）占菌株总数的34%。总共检测到10株（7%）产毒菌株：2株LT阳性、2株VT1阳性EntHly阳性、4株VT1阳性EntHly阴性、1株CNF1阳性Hly阳性和1株CNF2阳性。在西班牙兽医诊所最常用的抗生素组（四环素、链霉素、磺胺嘧啶、氨苄青霉素、卡那霉素、新霉素、氯霉素、甲氧苄啶和复方新诺明）中，抗生素耐药率最高。我们得出结论，从腹泻羔羊分离出的大肠杆菌菌株一般不产毒，且属于大量血清群。