Soh N, Nadal D, Schläpfer E, Bernstein J M, Ogra P L
Department of Pediatrics, State University of New York, Buffalo.
Ann Otol Rhinol Laryngol. 1992 Oct;101(10):848-53. doi: 10.1177/000348949210101008.
Adenoidal lymphocytes obtained from 43 subjects with serum antibodies to respiratory syncytial virus (RSV) were established in culture in vitro and analyzed for immunoglobulin (Ig) and RSV-specific antibody synthesis. Spontaneous synthesis of Ig was consistently observed in culture supernatants. The ratios of IgA to IgG and IgM to IgG in adenoidal lymphocyte culture supernatant were higher than in serum. In cell cultures stimulated with RSV or pokeweed mitogen, RSV antibody activity was detected in 25 of 43 (58.1%) for IgG, 5 of 43 (11.6%) for IgA, and 4 of 43 (9.3%) for IgM. Also, RSV-specific IgG was detected in some supernatants from unstimulated cultures. In seven cases the cultures of autologous tonsillar lymphocytes were also investigated. Autologous organs exhibited similar polyclonal Ig production and RSV-specific antibody synthesis. These observations demonstrate that both adenoids and palatine tonsils are continuously engaged in synthesis of local antibodies to viral pathogens available to the nasopharynx and respiratory mucosa.
从43名患有呼吸道合胞病毒（RSV）血清抗体的受试者中获取腺样体淋巴细胞，在体外进行培养，并分析其免疫球蛋白（Ig）和RSV特异性抗体的合成情况。在培养上清液中始终观察到Ig的自发合成。腺样体淋巴细胞培养上清液中IgA与IgG以及IgM与IgG的比值高于血清中的比值。在用RSV或商陆有丝分裂原刺激的细胞培养物中，43例中有25例（58.1%）检测到IgG的RSV抗体活性，43例中有5例（11.6%）检测到IgA的RSV抗体活性，43例中有4例（9.3%）检测到IgM的RSV抗体活性。此外，在未刺激培养物的一些上清液中也检测到了RSV特异性IgG。在7例病例中，还对自体扁桃体淋巴细胞培养物进行了研究。自体器官表现出类似的多克隆Ig产生和RSV特异性抗体合成。这些观察结果表明，腺样体和腭扁桃体都持续参与针对鼻咽和呼吸道黏膜中存在的病毒病原体的局部抗体合成。
