Müller M R, Grob P J, Hitzig W H
Int Arch Allergy Appl Immunol. 1977;54(3):269-80. doi: 10.1159/000231836.
Cord blood lymphocytes, generally believed to be immunologically uncommitted, were used for investigating the possible conversion by transfer factor (TF) of non-immune to immune lymphocytes in vitro. TF was prepared from pooled buffy coats of normal blood bank donors by ultrafiltration and lyophilization, and it ability to influence lymphocyte transformation was assessed by measuring increases in 3H-thymidine uptake into DNA after stimulation with purified protein derivative of tuberculin and/or streptokinase-streptodornase in 23 experiments with cord blood and 15 adult controls. TF stimulated cord blood lymphocytes nonspecifically when added to the medium alone. In the presence of antigen, TF acted in a synergistic, antigen-dependent way with either amplifying or inhibitory effects in adult good responders. These effects were negligible in adult low responders and in cord blood. From our observations no specific conversion of lymphocytes by TF in vitro can be deduced.
脐血淋巴细胞通常被认为在免疫上尚未定向分化，被用于研究转移因子（TF）在体外能否使非免疫淋巴细胞转化为免疫淋巴细胞。TF是通过超滤和冻干从正常血库供血者的混合血沉棕黄层制备而来，在23例脐血实验和15例成人对照实验中，通过测量经结核菌素纯蛋白衍生物和/或链激酶-链道酶刺激后DNA中3H-胸腺嘧啶核苷摄取量的增加，来评估其影响淋巴细胞转化的能力。单独添加到培养基中时，TF可非特异性地刺激脐血淋巴细胞。在有抗原存在的情况下，TF以协同的、抗原依赖性方式起作用，对成年高反应者有增强或抑制作用。这些作用在成年低反应者和脐血中可忽略不计。根据我们的观察，无法推断出TF在体外能使淋巴细胞发生特异性转化。
