Dowrie R H, Ebling W F, Mandema J W, Stanski D R
Department of Anesthesia, Stanford University School of Medicine, CA 94305, USA.
J Chromatogr B Biomed Appl. 1996 Apr 12;678(2):279-88. doi: 10.1016/0378-4347(95)00475-0.
This paper describes a sensitive HPLC-electrochemical detection analytical method for determining the concentration of the intravenous anesthetic, propofol, in human or rat plasma or serum and a variety of rat tissues. Internal standard and drug are extracted from serum or plasma and other tissues with pentane. 2,6-tert.-Butylmethylphenol is used as internal standard. It includes a novel steam distillation procedure for separating the highly lipophilic propofol from skin and fat. The plasma/serum assay has a precision of 1-4% (C.V.) in the range 10 ng/ml to 1 microgram/ml and permits the assay of assay of 5 ng/ml from 0.1 ml of plasma/serum. The tissue procedure allows the estimation of 50 ng/g in 0.1 g of tissue for most of the major organs with less than 2% (C.V.) precision. This assay was used to measure propofol concentrations in plasma/serum and tissue samples in support of a project to develop a physiological pharmacokinetic model for propofol in the rat.
本文描述了一种灵敏的高效液相色谱 - 电化学检测分析方法，用于测定人或大鼠血浆、血清以及多种大鼠组织中静脉麻醉药丙泊酚的浓度。内标物和药物用戊烷从血清、血浆及其他组织中萃取出来。采用2,6 - 二叔丁基甲基苯酚作为内标物。该方法包含一种新型水蒸气蒸馏程序，用于从皮肤和脂肪中分离高亲脂性的丙泊酚。血浆/血清检测在10 ng/ml至1 μg/ml范围内的精密度为1 - 4%（变异系数），且允许从0.1 ml血浆/血清中检测出5 ng/ml的丙泊酚。组织检测程序能够以低于2%（变异系数）的精密度，在0.1 g大多数主要器官组织中估算出50 ng/g的丙泊酚含量。该检测方法用于测量血浆/血清和组织样本中的丙泊酚浓度，以支持一项关于建立大鼠体内丙泊酚生理药代动力学模型的项目。
