Zee K Y, Pang K M, Samaranayake L P, Attström R
Department of Periodontology and Public Health, Faculty of Dentistry, University of Hong Kong.
J Clin Periodontol. 1996 Apr;23(4):403-6. doi: 10.1111/j.1600-051x.1996.tb00564.x.
The aim of the this study was to estimate the % distribution of bacterial morphotypes of supragingival plaque in Chinese subjects after 3 days of no oral hygiene. 36 dental students, hygienists or dental surgery assistants (mean age: 24.3 years) were recruited. Prophylaxis and oral hygiene reinforcement were given 1 x a week for 3 weeks to obtain gingival health and a 3-day period without any plaque control started afterwards. At the end of the 3-day period, the plaque along the gingival margin of the upper right central incisor was removed and direct Gram stained smears were prepared for light microscopy. Bacteria were classified as gram-positive or gram-negative cocci, rods, filaments, fusiform organisms, spirilla or spirochetes. A differential count of 200 organisms from 3-6 microscopic fields was performed and the results expressed as a % of the organisms counted. Results from the 36 direct smears showed approximately 83% (range: 52%-96%) of the organisms to be gram-negative while gram-positive bacteria comprised only a minority of about 12% (range: 0.7%-43%). This result is remarkably different from previous reports from the West where gram-positive bacteria were the predominant bacterial types noted in early supragingival plaque. Further longitudinal studies involving both microscopy and bacteriological culture will be necessary to confirm this finding.
本研究的目的是评估中国受试者在3天未进行口腔卫生护理后，龈上菌斑细菌形态类型的百分比分布。招募了36名牙科学生、口腔保健员或牙科手术助手（平均年龄：24.3岁）。每周进行1次预防和口腔卫生强化护理，持续3周以获得牙龈健康，之后开始为期3天不进行任何菌斑控制的阶段。在3天阶段结束时，清除右上中切牙龈缘处的菌斑，并制备直接革兰氏染色涂片用于光学显微镜检查。细菌分为革兰氏阳性或革兰氏阴性球菌、杆菌、丝状菌、梭形菌、螺旋菌或螺旋体。对3至6个显微镜视野中的200个生物体进行分类计数，结果以所计数生物体的百分比表示。36份直接涂片的结果显示，约83%（范围：52%-96%）的生物体为革兰氏阴性，而革兰氏阳性菌仅占少数，约为12%（范围：0.7%-43%）。这一结果与西方此前的报道显著不同，在西方，革兰氏阳性菌是早期龈上菌斑中主要的细菌类型。需要进一步开展涉及显微镜检查和细菌培养的纵向研究来证实这一发现。
