Zee K Y, Samaranayake L P, Attström R
Department of Periodontology and Public Health, Faculty of Dentistry, University of Hong Kong.
J Clin Periodontol. 1996 Nov;23(11):1025-31. doi: 10.1111/j.1600-051x.1996.tb00532.x.
The aim of this study was to compare the predominant cultivable bacterial flora in supragingival plaque samples of Chinese "rapid" and "slow" plaque formers, using the experimental gingivitis model. 11 Chinese subjects (5 "rapid" and 6 "slow" plaque formers) were selected from 49 healthy young adults. The selection was based on the plaque index on the buccal surfaces of all the canines, premolars and 1st molars after 3-days without plaque control. The 11 subjects began a 14-day on oral hygiene period after prophylaxis. Plaque samples were collected on day 1, day 3, day 7 and day 14 from the buccal surface of upper right canine, 2nd premolar, 1st premolar and 1st molar, respectively. The samples were then dispensed into a tryptic soy broth, and cultured anaerobically to obtain pure isolates which were then identified by conventional means. Results showed similar trends in plaque development in the two groups; gram-positive bacteria were the predominant cultivable species ("rapid": 71-37%; "slow": 53-63%) and gram-negative species increased in proportion to the plaque age ("rapid": 9-47%, "slow": 13-28%). "Rapid" plaque formers showed a statistically significant higher percentage of gram-negative rods (38%) than the "slow" group (17%) in the 14-day samples. The difference in the proportions of other groups of bacteria between the "rapid" and "slow" plaque formers were also found to be statistically significant using the MANOVA test (p = 0.0162). Most of the cultivable gram-negative rods belonged to Fusobacterium and Capnocytophaga species. Besides the quantitative difference observed clinically, there seems to be a qualitative difference in the cultivable flora between subjects with different rates of plaque formation.
本研究旨在利用实验性牙龈炎模型，比较中国“快速”和“缓慢”菌斑形成者龈上菌斑样本中主要的可培养细菌菌群。从49名健康年轻成年人中选取了11名中国受试者（5名“快速”菌斑形成者和6名“缓慢”菌斑形成者）。选择依据是在3天不进行菌斑控制后，所有犬齿、前磨牙和第一磨牙颊面的菌斑指数。这11名受试者在进行预防治疗后开始为期14天的口腔卫生期。分别在第1天、第3天、第7天和第14天，从右上颌犬齿、第二前磨牙、第一前磨牙和第一磨牙的颊面采集菌斑样本。然后将样本接种到胰蛋白胨大豆肉汤中，进行厌氧培养以获得纯分离株，随后通过传统方法进行鉴定。结果显示两组菌斑形成趋势相似；革兰氏阳性菌是主要的可培养菌种（“快速”组：71 - 37%；“缓慢”组：53 - 63%），革兰氏阴性菌的比例随菌斑龄增加（“快速”组：9 - 47%，“缓慢”组：13 - 28%）。在14天的样本中，“快速”菌斑形成者革兰氏阴性杆菌的百分比（38%）显著高于“缓慢”组（17%）。使用多变量方差分析检验发现，“快速”和“缓慢”菌斑形成者之间其他菌群比例的差异也具有统计学意义（p = 0.0162）。大多数可培养的革兰氏阴性杆菌属于梭杆菌属和二氧化碳嗜纤维菌属。除了临床上观察到的数量差异外，不同菌斑形成速率的受试者之间可培养菌群似乎存在质量差异。
