van der Hulst R W, Verheul S B, Weel J F, Gerrits Y, ten Kate F J, Dankert J, Tytgat G N
Department of Gastroenterology, Academic Medical Centre, Amsterdam, The Netherlands.
Eur J Clin Microbiol Infect Dis. 1996 Mar;15(3):211-5. doi: 10.1007/BF01591356.
Culture and histologic examination are considered "gold standard" methods for the detection of Helicobacter pylori, but discrepancies may occur with either method. Failure to detect Helicobacter pylori may be due to sampling error, inappropriate transport or culture media, or insufficient duration of the incubation period. Rates of detection of Helicobacter pylori by culture and histopathologic examination of gastric mucosal biopsy specimens were determined in 102 consecutive dyspeptic patients. In a separate group of 60 patients, rates of detection of Helicobacter pylori by culture of antral brushings and the length of incubation required in selective and nonselective culture media were studied. In the first group of 102 patients, the combination of culture and histologic examination detected 54 Helicobacter pylori-positive patients, whereas the separate techniques each detected 51 Helicobacter pylori-positive patients. In the second group of 60 patients evaluated by culture of antral brushings, the rate of detection of Helicobacter pylori was 25 of 60 and was similar for culture (25/60) and histologic examination (25/60). In the second group the length of incubation required to detect Helicobacter pylori was different for selective and nonselective media. In nonselective media, incubation of up to ten days was required to detect all Helicobacter pylori infections, whereas in selective media seven days was sufficient. Rates of detection of Helicobacter pylori by culture, histopathologic examination and culture from brushings were similar, whereas the combination of culture and histopathologic examination achieved a superior rate of detection. The incubation period required for the detection of Helicobacter pylori by culture was a minimum of seven days and was dependent on the culture medium used.
培养和组织学检查被认为是检测幽门螺杆菌的“金标准”方法，但两种方法都可能出现差异。未能检测到幽门螺杆菌可能是由于采样误差、运输或培养基不当，或培养期持续时间不足。对102例连续的消化不良患者的胃黏膜活检标本进行培养和组织病理学检查，以确定幽门螺杆菌的检出率。在另一组60例患者中，研究了通过胃窦刷检培养检测幽门螺杆菌的比率以及在选择性和非选择性培养基中所需的培养时间。在第一组102例患者中，培养和组织学检查相结合检测出54例幽门螺杆菌阳性患者，而单独的技术各检测出51例幽门螺杆菌阳性患者。在通过胃窦刷检培养评估的第二组60例患者中，幽门螺杆菌的检出率为60例中的25例，培养（25/60）和组织学检查（25/60）的检出率相似。在第二组中，检测幽门螺杆菌所需的培养时间在选择性和非选择性培养基中有所不同。在非选择性培养基中，需要长达十天的培养才能检测到所有幽门螺杆菌感染，而在选择性培养基中七天就足够了。通过培养、组织病理学检查和刷检培养检测幽门螺杆菌的比率相似，而培养和组织病理学检查相结合的检出率更高。通过培养检测幽门螺杆菌所需的培养期至少为七天，并且取决于所用的培养基。
