Canica M M, Nato F, du Merle L, Mazie J C, Baranton G, Postic D
Unit of Molecular and Medical Bacteriology, Pasteur Institute, Paris, France.
Scand J Infect Dis. 1993;25(4):441-8. doi: 10.3109/00365549309008525.
Borrelia isolates associated with Lyme borreliosis were previously divided into 3 genospecies, B. burgdorferi sensu stricto, B. garinii and group VS461, on the basis of DNA homology. B. burgdorferi sensu stricto and B. garinii were identified by monoclonal antibodies (MAbs), H3TS and D6 respectively, but no MAbs were available to identify group VS461. Two MAbs were produced, I 17.3 and J 8.3 which reacted with OspB and OspA proteins, respectively, of strains belonging to group VS461, which should be named B. afzelii sp. nov. 24 strains were assigned to B. afzelii sp. nov., 11 of them being isolated from skin lesions, 6 from acrodermatitis chronica atrophicans (ACA) and 5 from erythema chronicum migrans (ECM). Although quite unknown in the USA, ACA has frequently been reported in northern Europe where B. afzelii sp. nov. is commonly isolated. This study documents the involvement of B. afzelii sp. nov. as a specific aetiological agent of ACA.
先前根据DNA同源性，将与莱姆病疏螺旋体病相关的疏螺旋体分离株分为3个基因种，即狭义伯氏疏螺旋体、伽氏疏螺旋体和VS461组。狭义伯氏疏螺旋体和伽氏疏螺旋体分别通过单克隆抗体（MAb）H3TS和D6进行鉴定，但没有可用于鉴定VS461组的单克隆抗体。制备了两种单克隆抗体，I 17.3和J 8.3，它们分别与VS461组菌株的OspB和OspA蛋白发生反应，该组菌株应命名为阿氏疏螺旋体新种。24株菌株被归为阿氏疏螺旋体新种，其中11株从皮肤病变中分离得到，6株从慢性萎缩性肢端皮炎（ACA）中分离得到，5株从慢性游走性红斑（ECM）中分离得到。尽管在美国鲜为人知，但在北欧经常报告ACA病例，在那里通常分离出阿氏疏螺旋体新种。本研究证明阿氏疏螺旋体新种是ACA的一种特定病原体。
