Assous M V, Postic D, Paul G, Névot P, Baranton G
Laboratoire de Bactériologie, Faculté de Médecine Cochin, Paris, France.
Eur J Clin Microbiol Infect Dis. 1993 Apr;12(4):261-8. doi: 10.1007/BF01967256.
Sera of 52 Lyme borreliosis patients classified according to their clinical features were analysed by Western blot using as antigens Borrelia strains belonging to three recently described genomic species. The antibody response was demonstrated to be homologous within each genospecies. Serum reactivity was studied for each of the type strains Borrelia burgdorferi sensu stricto (strain B31T), Borrelia garinii (strain 20047T) and group VS461. Seven of 15 sera (46.6%) of patients with meningoradiculitis showed preferential reactivity with Borrelia garinii (strain 20047T), all of 8 sera (100%) of patients with acrodermatitis chronica atrophicans showed preferential reactivity with group VS461 (strain VS461) and 8 of 16 sera (50%) of patients with arthritis showed preferential reactivity with Borrelia burgdorferi sensu stricto (strain B31T). The presence of a strong response to OspA and OspB proteins of Borrelia burgdorferi sensu stricto was found only in this last group of patients. These results suggest that there are clinical implications of the recently described modifications in the taxonomy of Borrelia burgdorferi.
采用属于最近描述的三个基因组种的疏螺旋体菌株作为抗原，通过免疫印迹法分析了52例根据临床特征分类的莱姆病患者的血清。结果表明，每个基因种内的抗体反应具有同源性。研究了狭义伯氏疏螺旋体（菌株B31T）、伽氏疏螺旋体（菌株20047T）和VS461组各模式菌株的血清反应性。15例神经根脑膜炎患者血清中有7例（46.6%）对伽氏疏螺旋体（菌株20047T）表现出优先反应性，8例慢性萎缩性肢端皮炎患者血清全部（100%）对VS461组（菌株VS461）表现出优先反应性，16例关节炎患者血清中有8例（50%）对狭义伯氏疏螺旋体（菌株B31T）表现出优先反应性。仅在最后一组患者中发现对狭义伯氏疏螺旋体OspA和OspB蛋白有强烈反应。这些结果表明，最近描述的伯氏疏螺旋体分类学变化具有临床意义。
