几种从大鼠粪便中纯化微小隐孢子虫卵囊的技术的比较评估。
Comparative evaluation of several techniques for purification of Cryptosporidium parvum oocysts from rat feces.
作者信息
Suresh P, Rehg J E
机构信息
Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38101, USA.
出版信息
J Clin Microbiol. 1996 Jan;34(1):38-40. doi: 10.1128/jcm.34.1.38-40.1996.
Abstract
Three methods of isolating Cryptosporidium parvum oocysts from rat feces were evaluated. Oocysts were initially isolated by sucrose density gradient centrifugation. They were further purified by passage through a glass bead column or a Percoll gradient or by dialysis. Although oocysts recovered from the glass bead column and by dialysis were relatively free of fecal debris, only oocysts recovered from the Percoll gradient were free of bacteria. Recovered oocysts retained their antigenicity and infectivity. The ability to effectively recover oocysts from rat feces suggests that the laboratory rat may be a convenient substitute for ruminants in the propagation and maintenance of C. parvum oocysts for in vitro and in vivo use.
摘要
对从大鼠粪便中分离微小隐孢子虫卵囊的三种方法进行了评估。卵囊最初通过蔗糖密度梯度离心法分离。然后通过玻璃珠柱、Percoll梯度离心或透析进一步纯化。尽管从玻璃珠柱和透析法回收的卵囊相对不含粪便残渣，但只有通过Percoll梯度离心回收的卵囊无菌。回收的卵囊保留了其抗原性和感染性。能从大鼠粪便中有效回收卵囊表明，在繁殖和保存用于体外和体内实验的微小隐孢子虫卵囊方面，实验大鼠可能是反刍动物的便利替代物。
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