正常哺乳动物组织中胞壁酸的定性检测。
Qualitative detection of muramic acid in normal mammalian tissues.
作者信息
Sen Z, Karnovsky M L
出版信息
Infect Immun. 1984 Mar;43(3):937-41. doi: 10.1128/iai.43.3.937-941.1984.
Abstract
Rat tissues were extracted with 8% trichloroacetic acid, and the products were hydrolyzed with hydrochloric acid and purified. Fluorescamine derivatives were made and subjected to thin-layer chromatography; material with an Rf corresponding to the authentic muramyl derivative was obtained. It was oxidized with periodate, and the resulting formaldehyde was identified fluorimetrically. Alternatively, treatment with base released D-lactate (beta-elimination), which was identified fluorimetrically by reduction of NAD to NADH with D-lactate dehydrogenase. The data indicate that small muramyl compounds, presumably peptides of bacterial origin, are normally present in rat liver, brain, and kidney. The functions of muramyl compounds are suggested by much recent work.
摘要
用8%的三氯乙酸提取大鼠组织，产物用盐酸水解并纯化。制备荧光胺衍生物并进行薄层色谱分析；得到了与正品胞壁酰衍生物相对应的Rf值的物质。用高碘酸盐将其氧化，并用荧光法鉴定生成的甲醛。或者，用碱处理释放出D-乳酸（β-消除反应），用D-乳酸脱氢酶将NAD还原为NADH，并用荧光法鉴定D-乳酸。数据表明，大鼠肝脏、大脑和肾脏中通常存在小的胞壁酰化合物，推测是细菌来源的肽。近期的许多研究提示了胞壁酰化合物的功能。
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