鉴定一种新型隐球菌基因，该基因指导酿酒酵母隐性甘露醇脱氢酶基因的表达。
Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene.
作者信息
Perfect J R, Rude T H, Wong B, Flynn T, Chaturvedi V, Niehaus W
机构信息
Duke University Medical Center, Durham, North Carolina, USA.
出版信息
J Bacteriol. 1996 Sep;178(17):5257-62. doi: 10.1128/jb.178.17.5257-5262.1996.
Abstract
The Mtl gene from Cryptococcus neoformans, which confers the ability of Saccharomyces cerevisiae Sc4l YJO to grow on mannitol with substantial NAD-dependent mannitol dehydrogenase activity, was identified. Purifications and characterizations of this enzyme show that it is found in polyploid strain BB1, and the peptide sequence of the enzyme helped identify the saccharomyces gene encoding this mannitol dehydrogenase activity. On the other hand, the Mtl gene of C. neoformans encodes a 346-amino-acid protein which is not mannitol dehydrogenase but a regulatory element which is active in a heterologous fungus.
摘要
已鉴定出新型隐球菌的Mtl基因，该基因赋予酿酒酵母Sc4l YJO在甘露醇上生长的能力，并具有大量依赖NAD的甘露醇脱氢酶活性。对这种酶的纯化和特性分析表明，它存在于多倍体菌株BB1中，该酶的肽序列有助于鉴定编码这种甘露醇脱氢酶活性的酿酒酵母基因。另一方面，新型隐球菌的Mtl基因编码一种346个氨基酸的蛋白质，它不是甘露醇脱氢酶，而是一种在异源真菌中具有活性的调控元件。
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