钾缺乏胁迫对拟南芥精氨酸脱羧酶的调控
Regulation of Arabidopsis thaliana (L.) Heynh Arginine decarboxylase by potassium deficiency stress.
作者信息
Watson M B, Malmberg R L
机构信息
Botany Department, University of Georgia, Athens 30602-7271, USA.
出版信息
Plant Physiol. 1996 Aug;111(4):1077-83. doi: 10.1104/pp.111.4.1077.
Abstract
Arginine decarboxylase (ARGdc) is the first enzyme in one of the two pathways to putrescine in plants. ARGdc enzyme activity has been shown to be induced by many environmental factors, including potassium deficiency stress. We investigated the mechanism for induction of ARGdc activity during potassium deficiency stress in Arabidopsis thaliana (L.) Heynh. We show that A. thaliana responds to potassium deficiency stress by increasing ARGdc activity by up to 10-fold over unstressed plants with a corresponding increase in putrescine levels of up to 20-fold. Spermidine and spermine levels do not increase proportionately. Northern analysis showed no increase in ARGdc mRNA levels correlated with the increase in ARGdc enzyme activity. Western analysis revealed that there was no difference between ARGdc protein levels in stressed plants compared with controls. The increase in ARGdc enzyme activity due to potassium deficiency stress does not appear to involve changes in mRNA or protein abundance.
摘要
精氨酸脱羧酶（ARGdc）是植物中两条腐胺合成途径之一的首个酶。ARGdc酶活性已被证明受包括钾缺乏胁迫在内的多种环境因素诱导。我们研究了拟南芥在钾缺乏胁迫期间ARGdc活性诱导的机制。我们发现，拟南芥对钾缺乏胁迫的响应是将ARGdc活性提高至未受胁迫植株的10倍，腐胺水平相应提高至20倍。亚精胺和精胺水平未按比例增加。Northern分析表明，ARGdc mRNA水平的增加与ARGdc酶活性的增加不相关。Western分析显示，与对照相比，胁迫植株中ARGdc蛋白水平没有差异。钾缺乏胁迫导致的ARGdc酶活性增加似乎不涉及mRNA或蛋白质丰度的变化。
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