Kaur H, Halliwell B
Neurodegenerative Disease Research Centre, University of London, King's College, U.K.
Biochem J. 1996 Aug 15;318 ( Pt 1)(Pt 1):21-3. doi: 10.1042/bj3180021.
Oxidative DNA damage is thought to be an important contributor to cancer development and to be affected by dietary constituents, so its accurate measurement is important. DNA methylation is recognized as an important mechanism affecting gene expression. In the present paper we describe an HPLC-with-electrochemical-detection procedure that allows rapid and sensitive measurement of four oxidized (2,6-diamino-4-hydroxy-5-formamidopyrimidine, 5-hydroxyuracil, 8-hydroxyguanine, 8-hydroxyadenine) and three methylated (7-methylguanine, 1-methylguanine, O6-methylguanine) bases in acid hydrolysates of DNA. Guanine was also detected, but was clearly separated from the other bases.
氧化DNA损伤被认为是癌症发展的一个重要因素，并且会受到饮食成分的影响，因此对其进行准确测量很重要。DNA甲基化被公认为是影响基因表达的重要机制。在本文中，我们描述了一种采用电化学检测的高效液相色谱法，该方法能够快速、灵敏地测定DNA酸水解产物中的四种氧化碱基（2,6-二氨基-4-羟基-5-甲酰胺基嘧啶、5-羟基尿嘧啶、8-羟基鸟嘌呤、8-羟基腺嘌呤）和三种甲基化碱基（7-甲基鸟嘌呤、1-甲基鸟嘌呤、O6-甲基鸟嘌呤）。鸟嘌呤也能被检测到，并且与其他碱基能够清晰分离。
