Kurhan Faruk, Alp Hamit Hakan
Departments of Psychiatry, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey.
Departments of Biochemistry, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey.
Clin Psychopharmacol Neurosci. 2021 Nov 30;19(4):731-738. doi: 10.9758/cpn.2021.19.4.731.
Insufficient number of oxidative stress studies have been conducted in patients with adult attention deficit hyperactivity disorder (ADHD). The objective of the current study is to examine the thiol/disulfide homeostasis as well as oxidative DNA damage levels in adult ADHD patients and to compare them with the results of healthy control subjects.
The study was inclusive of forty-nine patients who were diagnosed with adult ADHD, as well as thirty-three healthy volunteers to be used as the control group. The diagnosis of the patients was conducted according to the DSM-5 diagnostic criteria. Blood were stored under appropriate laboratory conditions. For the purpose of detecting the oxidative DNA damage level, an extraction of genomic DNA from leukocytes was carried out, and furthermore the levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), apart from deoxyguanosine, were measured accordingly.
Total thiol and the native thiol levels were observed to be statistically lower in adult ADHD patients as compared to the subjects in the healthy control group ( = 0.001). It was observed that the disulfide levels were higher in adult ADHD patients as compared to the healthy control subjects ( = 0.001). In addition, the levels of 8-OHdG, which are considered as a marker for assessing DNA damage, were found to be significantly lower in the control group as compared to the adult ADHD patients ( = 0.001).
It was observed that the thiol/disulfide homeostasis had shifted towards disulfide, and 8-OHdG levels were increased in adult ADHD patients.
针对成人注意力缺陷多动障碍（ADHD）患者开展的氧化应激研究数量不足。本研究的目的是检测成人ADHD患者的硫醇/二硫键稳态以及氧化性DNA损伤水平，并将其与健康对照者的结果进行比较。
本研究纳入了49例被诊断为成人ADHD的患者，以及33名健康志愿者作为对照组。根据《精神疾病诊断与统计手册》第5版（DSM-5）诊断标准对患者进行诊断。血液在适当的实验室条件下储存。为检测氧化性DNA损伤水平，从白细胞中提取基因组DNA，并相应测量除脱氧鸟苷外的8-羟基-2'-脱氧鸟苷（8-OHdG）水平。
与健康对照组相比，成人ADHD患者的总硫醇和天然硫醇水平在统计学上较低（P = 0.001）。观察到成人ADHD患者的二硫键水平高于健康对照者（P = 0.001）。此外，作为评估DNA损伤标志物的8-OHdG水平在对照组中显著低于成人ADHD患者（P = 0.001）。
观察到成人ADHD患者的硫醇/二硫键稳态已向二硫键转移，且8-OHdG水平升高。
