Friedland J S, Hartley J C, Hartley C G, Shattock R J, Griffin G E
Department of Infectious Diseases and Bacteriology, Royal Postgraduate Medical School, London, UK.
Trans R Soc Trop Med Hyg. 1996 Mar-Apr;90(2):199-203. doi: 10.1016/s0035-9203(96)90141-8.
The human immune response to tuberculosis is partly mediated by the proinflammatory cytokines tumour necrosis factor (TNF), interleukin (IL)-6, and IL-8. We investigated plasma concentrations of these cytokines before and after maximal lipopolysaccharide stimulation ex vivo of whole blood leucocytes from Zambian patients. 32 patients with non-fatal tuberculosis, 25 of whom were seropositive for human immunodeficiency virus (HIV), were followed for 9 months. Patients were assessed at presentation to hospital (visit A), after 2 months' antimycobacterial therapy (visit B), and when chemotherapy was completed (visit C). Between visits A and B, patients regained weight (P = 0.03) and became less anaemic (P = 0.0001). At visit B, haemoglobin concentration remained lower in HIV seropositive patients (P = 0.001) and the erythrocyte sedimentation rate (ESR), initially elevated in all patients, was higher in HIV seropositive patients (100 +/- 6 mm vs. 43 +/- 11 mm in 1 h in seronegative patients; P = 0.002). Plasma IL-8 concentrations were increased at visit C as was IL-8 secretion ex vivo (P < 0.0001 at all time points). Otherwise plasma cytokine levels and secretion ex vivo remained similar throughout the study. Concurrent HIV infection resulted in persistently decreased IL-6 secretions ex vivo although ESR remained high. In summary, after antibiotic therapy in vivo IL-8 secretion ex vivo increased, which supports other data suggesting that IL-8 has a role in immunity to tuberculosis.
人类对结核病的免疫反应部分由促炎细胞因子肿瘤坏死因子（TNF）、白细胞介素（IL）-6和IL-8介导。我们研究了赞比亚患者全血白细胞在体外最大脂多糖刺激前后这些细胞因子的血浆浓度。32例非致命性结核病患者，其中25例人类免疫缺陷病毒（HIV）血清学阳性，随访9个月。在患者入院时（A访视）、抗分枝杆菌治疗2个月后（B访视）以及化疗完成时（C访视）进行评估。在A访视和B访视之间，患者体重增加（P = 0.03）且贫血减轻（P = 0.0001）。在B访视时，HIV血清学阳性患者的血红蛋白浓度仍较低（P = 0.001），所有患者最初均升高的红细胞沉降率（ESR）在HIV血清学阳性患者中更高（1小时内为100±6 mm，血清学阴性患者为43±11 mm；P = 0.002）。在C访视时血浆IL-8浓度升高，体外IL-8分泌也升高（所有时间点P < 0.0001）。否则，在整个研究过程中血浆细胞因子水平和体外分泌保持相似。并发HIV感染导致体外IL-6分泌持续减少，尽管ESR仍很高。总之，体内抗生素治疗后体外IL-8分泌增加，这支持了其他表明IL-8在结核病免疫中起作用的数据。
