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New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon.适用于革兰氏阴性菌基因替换的新型可移动载体及其在野油菜黄单胞菌野油菜致病变种胶操纵子3'端定位中的应用
Appl Environ Microbiol. 1999 Jan;65(1):278-82. doi: 10.1128/AEM.65.1.278-282.1999.
2
Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.野油菜黄单胞菌野油菜致病变种黄原胶操纵子的启动子分析，该操纵子指导黄原胶多糖的生物合成。
J Bacteriol. 1996 Jul;178(14):4313-8. doi: 10.1128/jb.178.14.4313-4318.1996.
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本文引用的文献

1
Xanthan gum biosynthesis and application: a biochemical/genetic perspective.黄原胶的生物合成与应用：生化/遗传学视角
Appl Microbiol Biotechnol. 1998 Aug;50(2):145-52. doi: 10.1007/s002530051269.
2
Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence.野油菜黄单胞菌野油菜致病变种的胶突变体：对黄原胶生物合成及植物致病性的影响
J Bacteriol. 1998 Apr;180(7):1607-17. doi: 10.1128/JB.180.7.1607-1617.1998.
3
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.空位BLAST和位置特异性迭代BLAST：新一代蛋白质数据库搜索程序。
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. doi: 10.1093/nar/25.17.3389.
4
An improved version of suicide vector pKNG101 for gene replacement in gram-negative bacteria.
Mol Microbiol. 1997 Jan;23(2):410-1. doi: 10.1046/j.1365-2958.1997.t01-1-00190.x.
5
Cyclic beta-(1,2)-glucan synthesis in Rhizobiaceae: roles of the 319-kilodalton protein intermediate.根瘤菌科中环状β-(1,2)-葡聚糖的合成：319千道尔顿蛋白质中间体的作用。
J Bacteriol. 1996 Oct;178(20):6043-8. doi: 10.1128/jb.178.20.6043-6048.1996.
6
Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.野油菜黄单胞菌野油菜致病变种黄原胶操纵子的启动子分析，该操纵子指导黄原胶多糖的生物合成。
J Bacteriol. 1996 Jul;178(14):4313-8. doi: 10.1128/jb.178.14.4313-4318.1996.
7
Expression of sialic acid and polysialic acid in serogroup B Neisseria meningitidis: divergent transcription of biosynthesis and transport operons through a common promoter region.B群脑膜炎奈瑟菌中唾液酸和多唾液酸的表达：生物合成和转运操纵子通过共同启动子区域的不同转录
J Bacteriol. 1996 Jul;178(14):4052-9. doi: 10.1128/jb.178.14.4052-4059.1996.
8
Location of a second O-acetyl group in xanthan gum by the reductive-cleavage method.通过还原裂解法确定黄原胶中第二个O-乙酰基的位置
Carbohydr Res. 1993 Mar 17;241:321-6. doi: 10.1016/0008-6215(93)80123-v.
9
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum.源自大肠杆菌质粒pK18和pK19的小型可移动多用途克隆载体：谷氨酸棒杆菌染色体中特定缺失的筛选
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10
Cloning of a cDNA encoding 3-ketoacyl-acyl carrier protein synthase III from Arabidopsis.从拟南芥中克隆编码 3-酮脂酰-酰基载体蛋白合酶 III 的 cDNA
Plant Physiol. 1994 Oct;106(2):801-2. doi: 10.1104/pp.106.2.801.

适用于革兰氏阴性菌基因替换的新型可移动载体及其在野油菜黄单胞菌野油菜致病变种胶操纵子3'端定位中的应用

New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon.

作者信息

Katzen F, Becker A, Ielmini M V, Oddo C G, Ielpi L

机构信息

Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, UBA, and CONICET, 1405 Buenos Aires, Argentina.

出版信息

Appl Environ Microbiol. 1999 Jan;65(1):278-82. doi: 10.1128/AEM.65.1.278-282.1999.

DOI:10.1128/AEM.65.1.278-282.1999
PMID:9872790
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC91013/
Abstract

We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan.

摘要

我们描述了在革兰氏阴性菌的定点标记交换诱变中直接选择双交换事件的有用载体。这些载体含有gusA标记基因，可提供比色筛选以鉴定携带那些序列的细菌。通过定位参与黄原胶生物合成的野油菜黄单胞菌 gum 操纵子的3' 末端，展示了这些载体的适用性。