Ye W C, Ji N N, Zhao S X, Liu J H, Ye T, McKervey M A, Stevenson P
Department of Phytochemistry, China Pharmaceutical University, Nanjing, China.
Phytochemistry. 1996 Jun;42(3):799-802. doi: 10.1016/0031-9422(96)00043-x.
A new lupane type triterpenic acid, pulsatillic acid, and two new lupane type triterpenoid glycosides, pulsatilloside A and B, along with the known 23-hydroxybetulinic acid were isolated from the roots of Pulsatilla chinensis. Their structures were characterized as 3-oxo-23-hydroxy-lup-20(29)-en-28-oic acid, 3 beta, 23-dihydroxy-lup-20(29)-en-28-oic acid 3-O-alpha-L-arabinopyranoside and 3 beta, 23-dihydroxy-lup-20(29)-en-28-oic acid 28-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside on the basis of hydrolysis and spectral evidence including two-dimensional relay HOHAHA, one-dimensional multiple relay COSY and ROESY NMR techniques. Pulsatillic acid exhibited cytotoxic activities against P-388, Lewis lung carcinoma and human large-cell lung carcinoma.
从白头翁的根中分离出一种新的羽扇豆烷型三萜酸——白头翁酸，以及两种新的羽扇豆烷型三萜糖苷——白头翁苷A和白头翁苷B，还有已知的23-羟基桦木酸。根据水解和光谱证据，包括二维接力HOHAHA、一维多重接力COSY和ROESY NMR技术，它们的结构被表征为3-氧代-23-羟基-羽扇-20(29)-烯-28-酸、3β,23-二羟基-羽扇-20(29)-烯-28-酸3-O-α-L-阿拉伯吡喃糖苷和3β,23-二羟基-羽扇-20(29)-烯-28-酸28-O-β-D-吡喃葡萄糖基-(1→6)-β-D-吡喃葡萄糖苷。白头翁酸对P-388、Lewis肺癌和人肺大细胞癌表现出细胞毒活性。
