Iwahashi M, Muragaki Y, Ooshima A, Nakano R
Department of Obstetrics and Gynecology, Wakayama Medical College, Japan.
J Clin Endocrinol Metab. 1996 Aug;81(8):2925-9. doi: 10.1210/jcem.81.8.8768853.
To provide some insight into the etiology of spontaneous abortion, the expression of type IV collagen was investigated in human decidual tissues obtained after spontaneous abortion (n = 17) and normal pregnancy (n = 22). Indirect immunofluorescent staining was performed for type I, III, and IV collagen as well as laminin, and Northern blot analysis was conducted to assess the expression of messenger ribonucleic acid for the alpha 1(IV) chain. Immunohistochemical analysis did not reveal any significant differences between normal pregnancy and spontaneous abortion with respect to interstitial collagens (type I and III collagen) and laminin in the decidual tissue. However, although pericellular immunostaining for type IV collagen was recognized around the decidual cells in normal pregnancy, very weak or no staining was observed in spontaneous abortion. Northern blot analysis revealed that the decidual expression of messenger ribonucleic acid for the alpha 1(IV) chain was significantly reduced in spontaneous abortion compared to that in normal pregnancy (P < 0.001). These results suggest that type IV collagen might play an important role in the maintenance of pregnancy and that decreased expression of this collagen could be associated with spontaneous abortion.
为了深入了解自然流产的病因，对自然流产（n = 17）和正常妊娠（n = 22）后获取的人蜕膜组织中IV型胶原的表达进行了研究。对I、III和IV型胶原以及层粘连蛋白进行了间接免疫荧光染色，并进行Northern印迹分析以评估α1(IV)链信使核糖核酸的表达。免疫组织化学分析显示，在蜕膜组织的间质胶原（I型和III型胶原）和层粘连蛋白方面，正常妊娠和自然流产之间没有任何显著差异。然而，尽管在正常妊娠的蜕膜细胞周围可识别出IV型胶原的细胞周免疫染色，但在自然流产中观察到的染色非常弱或无染色。Northern印迹分析显示，与正常妊娠相比，自然流产中α1(IV)链信使核糖核酸的蜕膜表达显著降低（P < 0.001）。这些结果表明，IV型胶原可能在维持妊娠中起重要作用，并且这种胶原表达的降低可能与自然流产有关。
