用原子力显微镜测量人体血小板的粘弹性特性。
Measuring the viscoelastic properties of human platelets with the atomic force microscope.
作者信息
Radmacher M, Fritz M, Kacher C M, Cleveland J P, Hansma P K
机构信息
Department of Physics, University of California, Santa Barbara 93106, USA.
出版信息
Biophys J. 1996 Jan;70(1):556-67. doi: 10.1016/S0006-3495(96)79602-9.
We have measured force curves as a function of the lateral position on top of human platelets with the atomic force microscope. These force curves show the indentation of the cell as the tip loads the sample. By analyzing these force curves we were able to determine the elastic modulus of the platelet with a lateral resolution of approximately 100 nm. The elastic moduli were in a range of 1-50 kPa measured in the frequency range of 1-50 Hz. Loading forces could be controlled with a resolution of 80 pN and indentations of the platelet could be determined with a resolution of 20 nm.
我们使用原子力显微镜测量了作用于人类血小板表面横向位置的力曲线。这些力曲线显示了随着探针加载样品，细胞的压痕情况。通过分析这些力曲线，我们能够以约100纳米的横向分辨率确定血小板的弹性模量。在1至50赫兹的频率范围内测得的弹性模量在1至50千帕的范围内。加载力的控制分辨率可达80皮牛，血小板的压痕测定分辨率可达20纳米。
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