Nicklas B J, Rogus E M, Colman E G, Goldberg A P
Department of Medicine, University of Maryland School of Medicine, Baltimore, USA.
Am J Physiol. 1996 Jan;270(1 Pt 1):E72-8. doi: 10.1152/ajpendo.1996.270.1.E72.
This study determines whether there are regional differences in lipolysis and whether adipocyte lipolysis is associated with the degree of visceral adiposity and its metabolic complications in 32 obese (28-37 kg/m2), nondiabetic, postmenopausal women. In vitro lipolysis was measured in the basal state and after addition of epinephrine (Epi), Epi plus yohimbine, Epi plus propranolol, and N6,2'-O-dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) in abdominal (ABD) and gluteal (GLT) adipocytes. Upper body obese [UBO, waist-to-hip ratio (WHR) > or = 0.80, n = 19] women had a greater intra-abdominal fat area (IA, 199 +/- 50 vs. 142 +/- 28 cm2) and Epi-stimulated lipolysis (ABD: 1.60 +/- 1.10 vs. 0.95 +/- 0.54 mumol glycerol.10(6) cells-1.2h; GLT: 1.14 +/- 0.70 vs. 0.66 +/- 0.42 mumol glycerol.10(6) cells-1.2 h) than lower body obese (LBO, WHR < 0.80, n = 13) women. The UBO women also had lower high-density lipoprotein cholesterol (1.39 +/- 0.40 vs. 1.64 +/- 0.39 mmol/l, P < 0.05), higher plasma triglycerides (1.89 +/- 0.48 vs. 1.44 +/- 0.56 mmol/l, P < 0.05), and higher fasting insulin levels (154 +/- 57 vs. 118 +/- 33 pmol/l, P < 0.05) than LBO women. Basal, adrenergic receptor-mediated, and DBcAMP-stimulated lipolytic rates in ABD and GLT adipocytes were positively correlated with IA (r = 0.44-0.76, P < 0.05, n = 28). In both UBO and LBO women, Epi-stimulated lipolysis was higher (+30%, P < 0.05) in ABD than GLT adipocytes. These results show that, in postmenopausal women, visceral obesity is associated with increased rates of lipolysis in both ABD and GLT subcutaneous adipocytes. The findings also indicate that Epi-stimulated lipolysis is greater in ABD than GLT adipocytes regardless of fat distribution.
本研究旨在确定32名肥胖（体重指数28 - 37kg/m²）、非糖尿病、绝经后女性的脂肪分解是否存在区域差异，以及脂肪细胞脂肪分解是否与内脏肥胖程度及其代谢并发症相关。在基础状态下以及在腹部（ABD）和臀肌（GLT）脂肪细胞中添加肾上腺素（Epi）、Epi加育亨宾、Epi加普萘洛尔和N6,2'-O-二丁酰腺苷3',5'-环磷酸（DBcAMP）后，测量体外脂肪分解。上身肥胖[UBO，腰臀比（WHR）≥0.80，n = 19]女性的腹内脂肪面积更大（IA，199±50 vs. 142±28cm²），且Epi刺激的脂肪分解更多（ABD：1.60±1.10 vs. 0.95±0.54μmol甘油·10⁶细胞⁻¹·2h；GLT：1.14±0.70 vs. 0.66±0.42μmol甘油·10⁶细胞⁻¹·2h），高于下身肥胖（LBO，WHR < 0.80，n = 13）女性。与LBO女性相比，UBO女性的高密度脂蛋白胆固醇也更低（1.39±0.40 vs. 1.64±0.39mmol/L，P < 0.05），血浆甘油三酯更高（1.89±0.48 vs. 1.44±0.56mmol/L，P < 0.05），空腹胰岛素水平更高（154±57 vs. 118±33pmol/L，P < 0.05）。ABD和GLT脂肪细胞的基础、肾上腺素能受体介导和DBcAMP刺激的脂肪分解率与IA呈正相关（r = 0.44 - 0.76，P < 0.05，n = 28）。在UBO和LBO女性中，Epi刺激的脂肪分解在ABD脂肪细胞中均高于GLT脂肪细胞（+30%，P < 0.05）。这些结果表明，在绝经后女性中，内脏肥胖与ABD和GLT皮下脂肪细胞中脂肪分解率增加相关。研究结果还表明，无论脂肪分布如何，Epi刺激的脂肪分解在ABD脂肪细胞中均大于GLT脂肪细胞。
