Simmonds P, Mellor J, Craxi A, Sanchez-Tapias J M, Alberti A, Prieto J, Colombo M, Rumi M G, Lo Iacano O, Ampurdanes-Mingall S, Forns-Bernhardt X, Chemello L, Civeira M P, Frost C, Dusheiko G
Department of Medical Microbiology, University of Edinburgh Medical School, UK.
J Hepatol. 1996 May;24(5):517-24. doi: 10.1016/s0168-8278(96)80135-8.
BACKGROUND/AIMS: Different variants of hepatitis C virus might show different susceptibility to interferon alpha treatment, but it is important to understand whether this difference in sensitivity reflects an association with other factors, such as cirrhosis or age.
We have used an enzyme-linked immunosorbent hepatitis C virus typing assay based upon the detection of antibody in patient's era to type-specific NS-4 antigens to investigate the effect of hepatitis C virus type in 610 patients with chronic hepatitis C virus infection. The influence of viral types and their interdependency with host factors were separately analyzed to establish which factors executed an independent effect on the probability of sustained response.
There was a marked difference in the distribution of hepatitis C virus types with age: infection with type 3 was more common in younger patients. The distribution of hepatitis C virus type with age is accounted for by differences in risk-factors for infection in different age groups. The frequency of cirrhosis increased markedly with age. Even after standardization for age, center and the presence of cirrhosis, viral type was strongly related to the outcome of infection.
Our data suggest that enzyme-linked immunosorbent hepatitis C virus typing could assist in patient selection for interferon treatment to improve sustained response rates. Together with measurement of viral load, hepatitis C virus typing may serve to indicate the probability of response in patients with chronic hepatitis C, and to elucidate antiviral mechanisms in the disease. The serotyping assay provides a relatively inexpensive screening method to determine the infecting hepatitis C virus type, which could facilitate therapeutic decisions and strategies in patients with chronic hepatitis C.
背景/目的：丙型肝炎病毒的不同变体可能对α干扰素治疗表现出不同的敏感性，但了解这种敏感性差异是否与其他因素（如肝硬化或年龄）相关很重要。
我们使用了一种基于检测患者血清中针对特定NS-4抗原的抗体的酶联免疫吸附丙型肝炎病毒分型检测方法，来研究丙型肝炎病毒类型对610例慢性丙型肝炎病毒感染患者的影响。分别分析病毒类型的影响及其与宿主因素的相互依赖性，以确定哪些因素对持续应答的概率产生独立影响。
丙型肝炎病毒类型的分布随年龄有显著差异：3型感染在年轻患者中更常见。丙型肝炎病毒类型随年龄的分布是由不同年龄组感染风险因素的差异造成的。肝硬化的发生率随年龄显著增加。即使在对年龄、中心和肝硬化的存在进行标准化后，病毒类型仍与感染结果密切相关。
我们的数据表明，酶联免疫吸附丙型肝炎病毒分型可有助于选择接受干扰素治疗的患者，以提高持续应答率。与病毒载量测量一起，丙型肝炎病毒分型可用于指示慢性丙型肝炎患者的应答概率，并阐明该疾病的抗病毒机制。血清分型检测提供了一种相对廉价的筛查方法，以确定感染的丙型肝炎病毒类型，这有助于慢性丙型肝炎患者的治疗决策和策略制定。
