Takano Y, Saegusa M, Masuda M, Mikami T, Okayasu I
Department of Pathology, Kitasato University School of Medicine, Kanagawa, Japan.
J Cancer Res Clin Oncol. 1997;123(7):395-401. doi: 10.1007/BF01240123.
In order to clarify the effects of Epstein-Barr virus (EBV) infection on apoptosis and proliferative activity of non-Hodgkin's lymphoma, 135 Japanese lymphoma cases were investigated for the presence of viral RNA and its correlation with bcl-2 protein (Bcl-2) expression. In addition, the role of EBV in lymphoma-genesis was also studied in terms of EBV genotyping and specific deletion in the gene for the latent membrane protein 1 (LMP-1). EBER-1 RNA in situ hybridization revealed EBV in 18 cases (13.3%), comprising 12 of 44 T cell (27.3%) and 6 of 91 B cell (6.6%) lymphomas. Type A EBV was found in all 18 cases (100%), and 17 of the 17 (100%) evaluable cases showed a 30-bp deletion within the 3' end of LMP-1. Comparison of apoptotic indices (AI), assessed by DNA nick-end labelling, and proliferative activity, estimated in terms of Ki-67 labelling and mitotic indices (KI and MI), demonstrated an overall correlation among AI, KI and MI increases in association with Bcl-2 negativity, indicating a close relation between apoptosis and proliferation. EBV-positive cases showed significantly elevated AI values, independent of Bcl-2 positivity, with no change in KI and MI. These results indicate that EBV in Japanese non-Hodgkin's lymphomas is exclusively of type A with a specific deletion in LMP-1 and that it tends to be present in T cell lymphomas. Moreover, EBV up-regulates apoptosis without any relation to Bcl-2 expression and exerts only minor effects on proliferation.
为阐明爱泼斯坦-巴尔病毒（EBV）感染对非霍奇金淋巴瘤凋亡及增殖活性的影响，对135例日本淋巴瘤病例进行了病毒RNA检测及其与bcl-2蛋白（Bcl-2）表达相关性的研究。此外，还从EBV基因分型及潜伏膜蛋白1（LMP-1）基因的特异性缺失方面研究了EBV在淋巴瘤发生中的作用。EBER-1 RNA原位杂交显示18例（13.3%）存在EBV，其中44例T细胞淋巴瘤中有12例（27.3%），91例B细胞淋巴瘤中有6例（6.6%）。所有18例（100%）均为A型EBV，17例可评估病例中的17例（100%）在LMP-1的3'端有30bp缺失。通过DNA缺口末端标记评估的凋亡指数（AI）与根据Ki-67标记和有丝分裂指数（KI和MI）估算的增殖活性进行比较，结果表明AI、KI和MI升高与Bcl-2阴性总体相关，提示凋亡与增殖之间存在密切关系。EBV阳性病例的AI值显著升高，与Bcl-2阳性无关，KI和MI无变化。这些结果表明，日本非霍奇金淋巴瘤中的EBV均为A型，LMP-1有特异性缺失，且倾向于存在于T细胞淋巴瘤中。此外，EBV上调凋亡，与Bcl-2表达无关，对增殖仅产生轻微影响。
