Barogi S, Baracca A, Parenti Castelli G, Bovina C, Formiggini G, Marchetti M, Solaini G, Lenaz G
Dipartimento di Biochimica G. Moruzzi, University of Bologna, Italy.
Mech Ageing Dev. 1995 Oct 13;84(2):139-50. doi: 10.1016/0047-6374(95)01640-6.
ATP hydrolase activity has been investigated in mitochondria from liver, heart, and skeletal muscle from adult (6 months) and aged (24 months) rats. No significant changes in total ATPase activity were observed in the three tissues, but the oligomycin sensitivity was slightly decreased in heart mitochondria of aged rats. The bicarbonate-induced stimulation of hydrolytic activity was somewhat decreased in mitochondria from aged rats, particularly in liver. No significant change was observed in ATPase activity after release of the endogenous inhibitor protein, IF1. It is concluded that no activity changes to be directly ascribed to the catalytic sector F1 of the enzyme occur upon ageing, but it cannot be excluded that changes in the membrane sector F0 occur as a consequence of mtDNA mutations.
已对成年（6个月）和老年（24个月）大鼠肝脏、心脏和骨骼肌线粒体中的ATP水解酶活性进行了研究。在这三种组织中未观察到总ATP酶活性有显著变化，但老年大鼠心脏线粒体中的寡霉素敏感性略有降低。老年大鼠线粒体中，尤其是肝脏线粒体中，碳酸氢盐诱导的水解活性刺激有所降低。释放内源性抑制蛋白IF1后，ATP酶活性未观察到显著变化。结论是，衰老过程中不会发生直接归因于该酶催化部分F1的活性变化，但不能排除由于线粒体DNA突变导致膜部分F0发生变化。
