空肠弯曲菌耐热血清型的综合核糖体分型方案
Comprehensive ribotyping scheme for heat-stable serotypes of Campylobacter jejuni.
作者信息
Fitzgerald C, Owen R J, Stanley J
机构信息
National Collection of Type Cultures, Central Public Health Laboratory, London, United Kingdom.
出版信息
J Clin Microbiol. 1996 Feb;34(2):265-9. doi: 10.1128/jcm.34.2.265-269.1996.
Abstract
Strains from diverse sources belonging to all 47 heat-stable Penner serotypes of Campylobacter jejuni were examined for polymorphism around the 16S rRNA genes. Penner serotype reference strains and a group of nonserotypeable isolates were included in the study. Complete typeability was obtained; 30 distinct PstI and 42 HaeIII polymorphisms were found. Three bands were detected in almost all strains with these enzymes, confirming that three copies of the 16S rRNA gene are typical for C.jejuni. By combination of the two enzyme polymorphisms, 77 16S ribotypes were defined among the 261 strains analyzed. With two exceptions, no specific association was observed between these ribotypes and heat-stable serotypes. Nine serotypes were homogeneous with respect to the 16S ribotype. Most nonserotypeable strains belonged to ribotypes defined elsewhere in the study. The 16S ribotypes of C.jejuni described here were not found in strains of Campylobacter coli, and vice versa.
摘要
对来自不同来源、属于空肠弯曲菌47种热稳定彭纳血清型的菌株进行了16S rRNA基因周围多态性的检测。研究纳入了彭纳血清型参考菌株和一组不可分型的分离株。获得了完全可分型性；发现了30种不同的PstI多态性和42种HaeIII多态性。用这些酶在几乎所有菌株中检测到三条带，证实16S rRNA基因的三个拷贝是空肠弯曲菌的典型特征。通过两种酶多态性的组合，在分析的261株菌株中定义了77种16S核糖体分型。除两个例外，这些核糖体分型与热稳定血清型之间未观察到特定关联。9种血清型在16S核糖体分型方面是同质的。大多数不可分型菌株属于在该研究其他地方定义的核糖体分型。这里描述的空肠弯曲菌的16S核糖体分型在大肠弯曲菌菌株中未发现，反之亦然。
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