Uchiyama T, Zhao L, Yan Y, Uchida T
Department of Virology, School of Medicine, University of Tokushima, Japan.
Microbiol Immunol. 1995;39(12):951-7. doi: 10.1111/j.1348-0421.1995.tb03298.x.
Cross-reactivity between Rickettsia japonica and R. typhi was observed by immunofluorescence tests using sera from patients with Oriental spotted fever (OSF), from whom the causative agent was isolated and identified as R. japonica. Western immunoblotting with these sera revealed that only the 120-kilodalton surface polypeptide, i.e., rickettsial outer membrane protein (rOmp) B, has a common antigenicity with the 105-kilodalton surface polypeptide of R. typhi. In some cases, antibodies specifically reactive with R. typhi were detected in acute-phase sera followed by a significant rise in titers, possibly because of an anamnestic response to a previous infection with an R. typhi-like agent; the sera retained reactivity to R. typhi even after absorption by a homologous strain. A lipopolysaccharide (LPS)-like antigen of R. typhi was found to be reactive with some sera of OSF patients. The ladder bands on Western immunoblot of rickettsial organisms were confirmed to be polysaccharide in nature, which was demonstrated by comparing them with the pattern of silver-stained gel of proteinase K-treated rickettsial specimens after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
利用从恙虫病患者血清进行免疫荧光试验观察到日本立克次氏体与伤寒立克次氏体之间的交叉反应，这些患者的病原体被分离并鉴定为日本立克次氏体。用这些血清进行的蛋白质免疫印迹分析表明，只有120千道尔顿的表面多肽，即立克次氏体外膜蛋白（rOmp）B，与伤寒立克次氏体的105千道尔顿表面多肽具有共同的抗原性。在某些情况下，急性期血清中检测到与伤寒立克次氏体特异性反应的抗体，随后滴度显著升高，这可能是由于对先前感染的类伤寒立克次氏体样病原体的回忆反应；即使在被同源菌株吸收后，这些血清仍保留对伤寒立克次氏体的反应性。发现伤寒立克次氏体的一种脂多糖（LPS）样抗原与一些恙虫病患者的血清发生反应。立克次氏体生物体蛋白质免疫印迹上的梯状条带经证实本质上是多糖，这是通过在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后将它们与蛋白酶K处理的立克次氏体标本的银染凝胶模式进行比较来证明的。
