Naz R K, Butler A
Department of Obstetrics and Gynecology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Am J Reprod Immunol. 1996 Jun;35(6):534-40. doi: 10.1111/j.1600-0897.1996.tb00054.x.
The purpose of this study was to investigate the levels of interleukin-6 and -8 (IL-6 and IL-8) in sera and cervical mucus of infertile (idiopathic and immunoinfertile) women and to compare the levels with those in age-matched normally cycling fertile women.
Levels of IL-6 and IL-8 were measured in the sera and cervical mucus of fertile and infertile women by the enzyme-linked immunosorbent assay (ELISA). A non-parametric Mann-Whitney test was used to evaluate significance between the means. Linear regression analysis of IL-6 and IL-8 concentrations in serum versus cervical mucus and with antisperm antibody titers was performed by condition as well as all groups together. P < 0.05 was considered significant.
Both IL-6 and IL-8 were detected in sera and cervical mucus of fertile as well as infertile groups. Although serum levels of IL-6 and IL-8 were significantly different between fertile and infertile groups, the differences were more pronounced in the cervical mucus samples. Cervical mucus of idiopathic and immunoinfertile women demonstrated significantly (P = 0.002 to < 0.0001) greater concentrations of IL-6 and IL-8 compared to fertile controls. In general, there was no significant correlation between the serum and cervical mucus IL-6 and IL-8 levels, whether analyzed by condition or all groups together.
Elevated levels of IL-6 and IL-8 in cervical mucus of infertile groups may play a role in etiology of idiopathic and immunologic infertility. These findings suggest that the measurements of cytokines (especially IL-6 and IL-8) in cervical mucus may provide a tool for specific diagnosis and possibly treatment of infertility, especially idiopathic infertility.
本研究旨在调查不孕（特发性和免疫性不孕）女性血清和宫颈黏液中白细胞介素-6和-8（IL-6和IL-8）的水平，并将其与年龄匹配的正常月经周期的可育女性的水平进行比较。
采用酶联免疫吸附测定法（ELISA）测量可育和不孕女性血清及宫颈黏液中IL-6和IL-8的水平。使用非参数曼-惠特尼检验评估均值之间的显著性。对血清与宫颈黏液中IL-6和IL-8浓度以及抗精子抗体滴度进行线性回归分析，按情况以及所有组一起进行分析。P < 0.05被认为具有显著性。
在可育组和不孕组的血清及宫颈黏液中均检测到IL-6和IL-8。虽然可育组和不孕组血清中IL-6和IL-8水平存在显著差异，但在宫颈黏液样本中差异更为明显。与可育对照组相比，特发性和免疫性不孕女性的宫颈黏液中IL-6和IL-8浓度显著更高（P = 0.002至< 0.0001）。总体而言，无论按情况分析还是所有组一起分析，血清和宫颈黏液中IL-6和IL-8水平之间均无显著相关性。
不孕组宫颈黏液中IL-6和IL-8水平升高可能在特发性和免疫性不孕的病因中起作用。这些发现表明，测量宫颈黏液中的细胞因子（尤其是IL-6和IL-8）可能为不孕，尤其是特发性不孕的特异性诊断和可能的治疗提供一种工具。
