Saitoh H, Takagaki K, Nakamura T, Munakata A, Yoshida Y, Endo M
Department of Biochemistry, Hirosaki University School of Medicine, Japan.
Dig Dis Sci. 1996 Sep;41(9):1768-74. doi: 10.1007/BF02088743.
Whole-gut lavage fluid, collected by administering an electrolyte lavage solution orally, was found to be an excellent and easily collectable source of abundant mucin. Furthermore, the biochemical features of the mucin from patients with ulcerative colitis and Crohn's disease were investigated. The mucin was separated into four fractions by Sepharose CL-4B, Sepharose CL-2B, and DEAE Sephacel chromatography. Compared with healthy subjects, the total yields of mucin from ulcerative colitis patients were low due to a deficiency of neutral mucin, whereas those from Crohn's disease patients were high, which was attributable mainly to high-molecular-weight mucin. The fucose and sulfate contents were low in ulcerative colitis, but only the former was low in Crohn's disease. The different biochemical features of the mucin obtained from whole gut lavage fluid appear to reflect mucosal pathological changes associated with inflammatory bowel disease.
通过口服电解质灌洗液收集的全肠道灌洗液体，被发现是丰富粘蛋白的优质且易于收集的来源。此外，还研究了溃疡性结肠炎和克罗恩病患者粘蛋白的生化特性。通过琼脂糖CL-4B、琼脂糖CL-2B和DEAE琼脂糖离子交换柱色谱法将粘蛋白分离为四个组分。与健康受试者相比，溃疡性结肠炎患者的粘蛋白总产量因中性粘蛋白缺乏而较低，而克罗恩病患者的粘蛋白总产量较高，这主要归因于高分子量粘蛋白。溃疡性结肠炎患者的岩藻糖和硫酸盐含量较低，但克罗恩病患者仅岩藻糖含量较低。从全肠道灌洗液体中获得的粘蛋白的不同生化特性似乎反映了与炎症性肠病相关的黏膜病理变化。
