Levy A M, Gleich G J, Sandborn W J, Tremaine W J, Steiner B L, Phillips S F
Division of Gastroenterology and Internal Medicine, Mayo Clinic Rochester, Minnesota 55905, USA.
Mayo Clin Proc. 1997 Feb;72(2):117-23. doi: 10.4065/72.2.117.
To explore the potential role of eosinophils in the pathogenesis of inflammatory bowel disease (IBD).
We measured the concentrations of eosinophil granule proteins-namely, major basic protein, eosinophil peroxidase, eosinophil cationic protein, and eosinophil-derived neurotoxin-in gut lavage fluid.
Ten healthy persons and 17 patients with IBD (9 with Crohn's disease and 8 with ulcerative colitis) underwent gut lavage. Each study subject submitted an early specimen when lavage effluent became liquid and a late specimen when the output became clear. The concentrations of the granule proteins were measured by immunoassay.
The median concentrations of eosinophil-derived neurotoxin and eosinophil cationic protein were significantly higher in patients with IBD than in control subjects for both early and late lavage specimens. Excretion of eosinophil peroxidase was also significantly higher in patients with IBD than in the healthy control subjects, but only in the early specimens. No differences were noted in the concentrations of any of the proteins between patients with ulcerative colitis and those with Crohn's disease.
Concentrations of eosinophil granule proteins were increased in whole gut lavage fluid from patients with IBD in comparison with healthy control subjects. These results encourage further studies of the role of eosinophils in the pathogenesis of IBD.
探讨嗜酸性粒细胞在炎症性肠病（IBD）发病机制中的潜在作用。
我们测量了肠道灌洗液中嗜酸性粒细胞颗粒蛋白的浓度，即主要碱性蛋白、嗜酸性粒细胞过氧化物酶、嗜酸性粒细胞阳离子蛋白和嗜酸性粒细胞衍生神经毒素。
10名健康人和17名IBD患者（9名克罗恩病患者和8名溃疡性结肠炎患者）接受肠道灌洗。每个研究对象在灌洗流出液变清时提交早期样本，在流出液变清时提交晚期样本。通过免疫测定法测量颗粒蛋白的浓度。
对于早期和晚期灌洗样本，IBD患者中嗜酸性粒细胞衍生神经毒素和嗜酸性粒细胞阳离子蛋白的中位浓度均显著高于对照组。IBD患者中嗜酸性粒细胞过氧化物酶的排泄量也显著高于健康对照组，但仅在早期样本中。溃疡性结肠炎患者和克罗恩病患者之间的任何蛋白质浓度均未发现差异。
与健康对照组相比，IBD患者全肠道灌洗液中嗜酸性粒细胞颗粒蛋白的浓度升高。这些结果鼓励进一步研究嗜酸性粒细胞在IBD发病机制中的作用。
