Crupper S S, Iandolo J J
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA.
Appl Environ Microbiol. 1996 Sep;62(9):3171-5. doi: 10.1128/aem.62.9.3171-3175.1996.
A novel antimicrobial agent from Staphylococcus aureus KSI1829, designated Bac1829, was purified by sequential steps of ammonium sulfate precipitation, Sephadex G-50 gel filtration chromatography, and hydrophobic interaction chromatography. Purified Bac1829 has a molecular mass of 6,418 +/- 2 Da. The peptide in heat stable, since full biological activity is retained after heating at 95 degrees C for 15 min, and it is destroyed by digestion with proteases. Amino acid sequence analysis revealed a high concentration of Ala and Gly residues, which respectively comprised 24 and 19% of the total amino acid content. Additionally, high levels of hydrophobic amino acids were present, accounting for the hydrophobic nature of Bac1829. Purified Bac1829 killed exponentially growing Corynebacterium renale in a dose-dependent manner by a bactericidal mode of action. A partial inhibitory spectrum analysis revealed that the following organisms were sensitive to the inhibitory activity of Bac1829: S. aureus RN4220, Streptococcus suis, Corynebacterium pseudotuberculosis, C. renale, Corynebacterium diptheriae, Haemophilus parasuis, Bordetella pertussis, Bordetella bronchoseptica, Moraxella bovis, and Pasteurella multocida.
一种来自金黄色葡萄球菌KSI1829的新型抗菌剂，命名为Bac1829，通过硫酸铵沉淀、Sephadex G - 50凝胶过滤色谱和疏水相互作用色谱等连续步骤进行纯化。纯化后的Bac1829分子量为6418±2道尔顿。该肽具有热稳定性，因为在95℃加热15分钟后仍保留全部生物活性，并且会被蛋白酶消化破坏。氨基酸序列分析显示Ala和Gly残基浓度较高，分别占总氨基酸含量的24%和19%。此外，存在高水平的疏水氨基酸，这解释了Bac1829的疏水性。纯化后的Bac以杀菌作用模式以剂量依赖方式杀死指数生长的肾棒状杆菌。部分抑菌谱分析表明，以下微生物对Bac1829的抑菌活性敏感：金黄色葡萄球菌RN4220、猪链球菌、伪结核棒状杆菌、肾棒状杆菌、白喉棒状杆菌、副猪嗜血杆菌、百日咳博德特氏菌、支气管败血博德特氏菌、牛莫拉菌和多杀性巴氏杆菌。
