Wegener H C, Andresen L O, Bille-Hansen V
National Veterinary Laboratory, Copenhagen, Denmark.
Can J Vet Res. 1993 Apr;57(2):119-25.
Staphylococcus hyicus strains with different phage types, plasmid profiles, and antibiotic resistance patterns were isolated from piglets with exudative epidermitis. The strains could be divided into virulent strains, producing exudative epidermitis, and avirulent strains, producing no dermal changes when injected in experimental piglets. The results showed that both virulent and avirulent strains were present simultaneously on diseased piglets. This constitutes a diagnostic problem. Concentrated culture supernatants from nine virulent strains injected in the skin of healthy piglets produced a crusting reaction in all piglets. Acanthosis was observed in the histopathological examination of the crustaceous skin. Concentrated culture supernatants from nine avirulent strains produced no macroscopic or microscopic skin changes. Protein profiles from all virulent strains and seven out of nine avirulent strains showed a high degree of protein band homology. An approximately 30 kDa protein present in all concentrated culture supernatants capable of producing skin changes, could not be detected in samples that did not produce skin changes. No other protein showed a similar association. It is concluded that crusting reaction of piglet skin is a suitable indicator of virulence in S. hyicus in relation to exudative epidermitis, and that virulent strains produce a 30 kDa protein, absent in concentrated culture supernatants from avirulent strains. This 30 kDa protein might be an exfoliative toxin.
从患有渗出性皮炎的仔猪中分离出具有不同噬菌体类型、质粒图谱和抗生素抗性模式的猪葡萄球菌菌株。这些菌株可分为产生渗出性皮炎的强毒株和注射到实验仔猪中不引起皮肤变化的无毒株。结果表明，患病仔猪身上同时存在强毒株和无毒株。这构成了一个诊断问题。将9株强毒株的浓缩培养上清液注射到健康仔猪皮肤中，所有仔猪均出现结痂反应。对结痂皮肤进行组织病理学检查时观察到棘皮症。9株无毒株的浓缩培养上清液未引起肉眼可见或显微镜下的皮肤变化。所有强毒株和9株无毒株中的7株的蛋白质谱显示出高度的蛋白条带同源性。在所有能够引起皮肤变化的浓缩培养上清液中存在的一种约30 kDa的蛋白质，在未引起皮肤变化的样本中未检测到。没有其他蛋白质显示出类似的关联。结论是，仔猪皮肤结痂反应是猪葡萄球菌与渗出性皮炎相关的毒力的合适指标，并且强毒株产生一种30 kDa的蛋白质，无毒株的浓缩培养上清液中不存在该蛋白质。这种30 kDa的蛋白质可能是一种剥脱毒素。
