Jiménez M, Máñez M, Hernández E
Departamento de Microbiología, Facultad de Ciencias Biológicas, Universitat de Valencia, Spain.
Int J Food Microbiol. 1996 Apr;29(2-3):417-21. doi: 10.1016/0168-1605(95)00073-9.
The production of zearalenone in corn by seven isolates of Fusarium belonging to F. graminearum, F. culmorum and F. oxysporum was investigated for different culture conditions (water activity, temperature and incubation time). Two isolates belonging to F. graminearum were the highest producers of zearalenone (5150 and 7100 mg/kg dry weight of culture medium). The culture conditions that gave the highest yields were a(w) = 0.97, 40 days of incubation and temperature conditions including 2 weeks at 28 degrees C followed by incubation at 12 degrees C. These conditions were also optimal for the two isolates of F. oxysporum but for the remaining isolates the maximum levels of zearalenone were obtained at room temperature and 30 days of incubation. The maximum rate of mycotoxin production was observed between day 15 and 30 for all strains tested. At a(w) = 0.95 the maximum yield of zearalenone was 9.3 mg/kg dry weight of culture medium, produced by an isolate of F. graminearum. At 37 degrees C zearalenone was not detectable under any of the conditions assayed.
研究了属于禾谷镰刀菌、燕麦镰刀菌和尖孢镰刀菌的7株镰刀菌在不同培养条件（水分活度、温度和培养时间）下玉米中玉米赤霉烯酮的产生情况。属于禾谷镰刀菌的2株菌株是玉米赤霉烯酮的最高产生菌（5150和7100毫克/千克干重培养基）。产生最高产量的培养条件是水分活度=0.97、培养40天以及温度条件，包括在28℃下培养2周，然后在12℃下培养。这些条件对尖孢镰刀菌的2株菌株也是最佳的，但对于其余菌株，在室温下培养30天可获得玉米赤霉烯酮的最高水平。在所测试的所有菌株中，在第15天至30天观察到霉菌毒素产生的最大速率。在水分活度=0.95时，由一株禾谷镰刀菌产生的玉米赤霉烯酮的最高产量为9.3毫克/千克干重培养基。在37℃下，在所测定的任何条件下均未检测到玉米赤霉烯酮。
