Verbrugge S J, Gommers D, Bos J A, Hansson C, Wollmer P, Bakker W H, Lachmann B
Department of Anesthesiology, Erasmus University Rotterdam, The Netherlands.
Crit Care Med. 1996 Sep;24(9):1518-23. doi: 10.1097/00003246-199609000-00015.
Pulmonary clearance of technetium-labeled human serum albumin was measured in order to investigate whether the surfactant layer is a rate-limiting factor for the permeability of the alveolar-capillary membrane for 99mTc-labeled albumin.
Prospective, randomized, controlled trial.
Research laboratory.
Nineteen white New Zealand adult rabbits.
Three groups of rabbits were studied: group 1 animals received natural surfactant after lung lavage; group 2 animals underwent lung lavage only; and group 3 animals were not lavaged and served as an untreated, healthy control group. All animals were ventilated with high pressures.
99mTc-labeled albumin was nebulized into the inspiratory line of the breathing circuit with an air jet nebulizer. The clearance measurements were then immediately started. Gamma camera images were obtained in 1-min frames for 120 mins and stored in a 64 x 64 image matrix in a computer. In group 1 animals, surfactant restored blood gases to near normal, and all animals except one had bi-exponential clearance curves. The half-life of the fast compartment was 35.9 +/- 6.4 mins, and the half-life of the slow compartment was 847.5 +/- 143.5 mins. All group 2 animals also had bi-exponential clearance curves of the tracer (the half-lives of the fast and slow compartments were 14.6 +/- 6.7 and 459.8 +/- 167 mins, respectively). The half-lives of both the fast (p < .01) and slow (p < .01) components were significantly different between groups 1 and 2. Group 3 had a mono-exponential half-life of 580 +/- 225 mins.
The use of 99mTc-human serum albumin as a tracer molecule is possible and feasible. The clearance of this tracer is, in part, determined by the integrity of the pulmonary surfactant system, as it is with 99mTc-diethylenetriamine pentaacetate.
通过测量锝标记的人血清白蛋白的肺清除率，以研究表面活性物质层是否是肺泡-毛细血管膜对99mTc标记白蛋白通透性的限速因素。
前瞻性、随机、对照试验。
研究实验室。
19只成年新西兰白兔。
研究分为三组：第1组动物在肺灌洗后接受天然表面活性物质；第2组动物仅接受肺灌洗；第3组动物不进行灌洗，作为未治疗的健康对照组。所有动物均接受高压通气。
用空气喷射雾化器将99mTc标记的白蛋白雾化至呼吸回路的吸气管道中。然后立即开始清除率测量。以1分钟为帧获取γ相机图像，持续120分钟，并存储在计算机的64×64图像矩阵中。在第1组动物中，表面活性物质使血气恢复至接近正常，除1只动物外，所有动物均具有双指数清除曲线。快速成分的半衰期为35.9±6.4分钟，慢速成分的半衰期为847.5±143.5分钟。所有第2组动物也具有示踪剂的双指数清除曲线（快速和慢速成分的半衰期分别为14.6±6.7和459.8±167分钟）。第1组和第2组之间快速成分（p<0.01）和慢速成分（p<0.01）的半衰期均有显著差异。第3组的单指数半衰期为580±225分钟。
使用99mTc-人血清白蛋白作为示踪分子是可行的。该示踪剂的清除率部分取决于肺表面活性物质系统的完整性，与99mTc-二乙三胺五乙酸的情况相同。
