Sattar A A, Haot J, Schulman C C, Wespes E
Department of Urology, University Clinics of Brussels, Belgium.
Br J Urol. 1996 Feb;77(2):266-70. doi: 10.1046/j.1464-410x.1996.83018.x.
To compare the staining properties of anti-desmin and anti-actin in cavernous biopsies and thus determine the optimal antibody for quantifying intracavernous smooth muscle content in the evaluation of erectile dysfunction.
Twenty-two penile biopsies were taken from five normal potent patients, seven with venous leak and 10 with arterial disease, with mean ages of 34.4, 48.8 and 54.8 years, respectively. Biopsies were sectioned and successive sections stained immunohistochemically with antibodies for actin or desmin. Image analysis was used to quantify the proportion of smooth muscle fibres in the cavernous tissue.
There was a significant difference (P = 0.0001) between the mean percentage of cavernous smooth muscle fibres in normal potent men, stained with anti-desmin (38.5%, SD 3.2) or anti-actin (45.2%, SD 3.2) and that in the venous group (anti-desmin 27.4%, SD 4.0%; anti-actin 34.2%, SD 5.3%) or the arteriogenic group (anti-desmin 23.7%, SD 4.4, anti-actin 28.9%, SD 4.9). There was no significant difference in the estimates with either staining method between the venogenic and arteriogenic patients. There was a direct relationship between the cavernous smooth muscle density estimated by anti-desmin and anti-actin staining (r = 0.96).
The anti-actin and anti-desmin staining methods gave equivalent results for discriminating between normal and impotent patients on the basis of the percentage of cavernous muscle fibres. However, anti-actin staining tended to be more sensitive than anti-desmin.
比较抗结蛋白和抗肌动蛋白在海绵体活检组织中的染色特性，从而确定在勃起功能障碍评估中用于量化海绵体内平滑肌含量的最佳抗体。
从5名性功能正常的患者、7名静脉性漏血患者和10名动脉性疾病患者中获取22份阴茎活检组织，他们的平均年龄分别为34.4岁、48.8岁和54.8岁。将活检组织切片，连续切片用抗肌动蛋白或抗结蛋白抗体进行免疫组织化学染色。采用图像分析技术量化海绵体组织中平滑肌纤维的比例。
正常性功能男性中，用抗结蛋白（38.5%，标准差3.2）或抗肌动蛋白（45.2%，标准差3.2）染色的海绵体平滑肌纤维平均百分比与静脉性组（抗结蛋白27.4%，标准差4.0%；抗肌动蛋白34.2%，标准差5.3%）或动脉源性组（抗结蛋白23.7%，标准差4.4，抗肌动蛋白28.9%，标准差4.9）之间存在显著差异（P = 0.0001）。静脉性和动脉源性患者之间，两种染色方法的估计值无显著差异。抗结蛋白和抗肌动蛋白染色估计的海绵体平滑肌密度之间存在直接关系（r = 0.96）。
基于海绵体肌纤维百分比，抗肌动蛋白和抗结蛋白染色方法在区分正常和阳痿患者方面得出了等效结果。然而，抗肌动蛋白染色往往比抗结蛋白染色更敏感。
