Stemler A
Biochim Biophys Acta. 1977 Jun 9;460(3):511-22. doi: 10.1016/0005-2728(77)90089-5.
Radioactive labelling techniques show that isolated broken chloroplasts can take up HCO3-in the dark. There are two pools of binding sites for this ion on, or within, the thylakoid membranes. A smaller, high affinity pool exists at a concentration of one HCO3-bound per 380-400 chlorophyll molecules. Removal of HCO3-bound in this pool requires special conditions and results in greater than 90% inhibition of oxygen evolution. The inhibition is fully reversed when HCO3-is added back. HCO3-bound in the small pool does not necessarily exchange with free HCO3-in the dark or in light. Evidence presented suggests that this site is very near the site of action of 3-(3,4-dichlorophenyl)-1,1-dimethyl urea. A second, much larger, pool of HCO3-binding sites also exists in a concentration approaching that of the bulk chlorophyll. These sites have a much lower affinity for HCO3-, and their function has not yet been determined.
放射性标记技术表明，分离出的破碎叶绿体在黑暗中能够吸收HCO₃⁻。在类囊体膜上或膜内存在两个该离子的结合位点池。一个较小的、高亲和力的池以每380 - 400个叶绿素分子结合一个HCO₃⁻的浓度存在。去除该池中结合的HCO₃⁻需要特殊条件，并且会导致氧气释放受到超过90%的抑制。当重新添加HCO₃⁻时，抑制作用会完全逆转。小池中结合的HCO₃⁻在黑暗或光照条件下不一定与游离的HCO₃⁻进行交换。所提供的数据表明，该位点非常接近3 -（3,4 - 二氯苯基）- 1,1 - 二甲基脲的作用位点。还存在第二个大得多的HCO₃⁻结合位点池，其浓度接近叶绿素的总体浓度。这些位点对HCO₃⁻的亲和力要低得多，其功能尚未确定。
