在酵母中，与HIS4基因座上游端粒序列插入相关的重组热点处，减数分裂特异性双链DNA断裂的精细结构图谱。
Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast.
作者信息
Xu F, Petes T D
机构信息
Department of Biology, University of North Carolina, Chapel Hill, 27599-3280, USA.
出版信息
Genetics. 1996 Jul;143(3):1115-25. doi: 10.1093/genetics/143.3.1115.
Abstract
Meiotic recombination in Saccharomyces cerevisiae is initiated by double-strand DNA breaks (DSBs). Using two approaches, we mapped the position of DSBs associated with a recombination hotspot created by insertion of telomeric sequences into the region upstream of HIS4. We found that the breaks have no obvious sequence specificity and localize to a region of approximately 50 bp adjacent to the telomeric insertion. By mapping the breaks and by studies of the exonuclease III sensitivity of the broken ends, we conclude that most of the broken DNA molecules have blunt ends with 3'-hydroxyl groups.
摘要
酿酒酵母中的减数分裂重组由双链DNA断裂（DSB）引发。我们采用两种方法，绘制了与通过将端粒序列插入HIS4上游区域而产生的重组热点相关的DSB位置。我们发现这些断裂没有明显的序列特异性，并且定位在与端粒插入相邻的大约50 bp区域内。通过绘制断裂位置以及对断裂末端的核酸外切酶III敏感性进行研究，我们得出结论，大多数断裂的DNA分子具有带有3'-羟基的平端。
相似文献
1
Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast.在酵母中，与HIS4基因座上游端粒序列插入相关的重组热点处，减数分裂特异性双链DNA断裂的精细结构图谱。
Genetics. 1996 Jul;143(3):1115-25. doi: 10.1093/genetics/143.3.1115.
2
Competition between adjacent meiotic recombination hotspots in the yeast Saccharomyces cerevisiae.酿酒酵母中相邻减数分裂重组热点之间的竞争
Genetics. 1997 Mar;145(3):661-70. doi: 10.1093/genetics/145.3.661.
3
Relationship between nuclease-hypersensitive sites and meiotic recombination hot spot activity at the HIS4 locus of Saccharomyces cerevisiae.酿酒酵母HIS4基因座上核酸酶超敏位点与减数分裂重组热点活性之间的关系。
Mol Cell Biol. 1996 May;16(5):2037-43. doi: 10.1128/MCB.16.5.2037.
4
Deletion of flanking ARS elements does not affect meiotic recombination at the HIS4 locus in yeast.侧翼自主复制序列元件的缺失不影响酵母中HIS4基因座的减数分裂重组。
Curr Genet. 1997 Feb;31(2):106-11. doi: 10.1007/s002940050182.
5
A 140-bp-long palindromic sequence induces double-strand breaks during meiosis in the yeast Saccharomyces cerevisiae.一段140个碱基对长的回文序列在酿酒酵母减数分裂过程中诱导双链断裂。
Genetics. 1997 Jul;146(3):835-47. doi: 10.1093/genetics/146.3.835.
6
Sequence non-specific double-strand breaks and interhomolog interactions prior to double-strand break formation at a meiotic recombination hot spot in yeast.酵母减数分裂重组热点处双链断裂形成之前的序列非特异性双链断裂及同源染色体间相互作用。
EMBO J. 1995 Oct 16;14(20):5115-28. doi: 10.1002/j.1460-2075.1995.tb00194.x.
7
Long palindromic sequences induce double-strand breaks during meiosis in yeast.长回文序列在酵母减数分裂过程中诱导双链断裂。
Mol Cell Biol. 2000 May;20(10):3449-58. doi: 10.1128/MCB.20.10.3449-3458.2000.
8
The nucleotide mapping of DNA double-strand breaks at the CYS3 initiation site of meiotic recombination in Saccharomyces cerevisiae.酿酒酵母减数分裂重组CYS3起始位点处DNA双链断裂的核苷酸定位
EMBO J. 1995 Sep 15;14(18):4589-98. doi: 10.1002/j.1460-2075.1995.tb00138.x.
9
Two different types of double-strand breaks in Saccharomyces cerevisiae are repaired by similar RAD52-independent, nonhomologous recombination events.酿酒酵母中两种不同类型的双链断裂通过类似的不依赖RAD52的非同源重组事件进行修复。
Mol Cell Biol. 1994 Feb;14(2):1293-301. doi: 10.1128/mcb.14.2.1293-1301.1994.
10
Control of meiotic recombination and gene expression in yeast by a simple repetitive DNA sequence that excludes nucleosomes.通过一个排除核小体的简单重复DNA序列来控制酵母中的减数分裂重组和基因表达。
Mol Cell Biol. 1999 Nov;19(11):7661-71. doi: 10.1128/MCB.19.11.7661.
引用本文的文献
1
Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis.Spo11与减数分裂中DNA双链断裂的形成
Genome Dyn Stab. 2008 Jan 1;2:81-123. doi: 10.1007/7050_2007_026.
2
A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation.多种因素的层级组合塑造了酵母减数分裂重组起始的全基因组拓扑结构。
Cell. 2011 Mar 4;144(5):719-31. doi: 10.1016/j.cell.2011.02.009.
3
Gel electrophoresis assays for analyzing DNA double-strand breaks in Saccharomyces cerevisiae at various spatial resolutions.用于在不同空间分辨率下分析酿酒酵母中DNA双链断裂的凝胶电泳测定法。
Methods Mol Biol. 2009;557:117-42. doi: 10.1007/978-1-59745-527-5_9.
4
Trans-regulation of mouse meiotic recombination hotspots by Rcr1.Rcr1对小鼠减数分裂重组热点的反式调控。
PLoS Biol. 2009 Feb 17;7(2):e36. doi: 10.1371/journal.pbio.1000036.
5
Sites of strong Rec12/Spo11 binding in the fission yeast genome are associated with meiotic recombination and with centromeres.裂殖酵母基因组中Rec12/Spo11强结合位点与减数分裂重组及着丝粒相关。
Chromosoma. 2008 Oct;117(5):431-44. doi: 10.1007/s00412-008-0159-3. Epub 2008 May 1.
6
Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae.对酿酒酵母减数分裂重组过程中形成的碱基错配和四碱基环的体内修复所涉及蛋白质的分析。
Genetics. 2006 Jul;173(3):1223-39. doi: 10.1534/genetics.106.055616. Epub 2006 May 15.
7
Infrequent co-conversion of markers flanking a meiotic recombination initiation site in Saccharomyces cerevisiae.酿酒酵母减数分裂重组起始位点侧翼标记的罕见共转换。
Genetics. 2005 Mar;169(3):1353-67. doi: 10.1534/genetics.104.036509. Epub 2005 Jan 16.
8
Hotspots of homologous recombination in the human genome: not all homologous sequences are equal.人类基因组中同源重组的热点：并非所有同源序列都是等同的。
Genome Biol. 2004;5(10):242. doi: 10.1186/gb-2004-5-10-242. Epub 2004 Sep 28.
9
Mouse strains with an active H2-Ea meiotic recombination hot spot exhibit increased levels of H2-Ea-specific DNA breaks in testicular germ cells.具有活跃的H2-Ea减数分裂重组热点的小鼠品系在睾丸生殖细胞中表现出H2-Ea特异性DNA断裂水平增加。
Mol Cell Biol. 2004 Feb;24(4):1655-66. doi: 10.1128/MCB.24.4.1655-1666.2004.
10
Signal and noise in bridging PCR.桥接PCR中的信号与噪声
BMC Biotechnol. 2002 Jul 18;2:13. doi: 10.1186/1472-6750-2-13.
本文引用的文献
1
Meiotic recombination hotspots.减数分裂重组热点
Annu Rev Genet. 1995;29:423-44. doi: 10.1146/annurev.ge.29.120195.002231.
2
Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.酿酒酵母减数分裂重组过程中异源双链体的物理检测。
Mol Cell Biol. 1993 Apr;13(4):2324-31. doi: 10.1128/mcb.13.4.2324-2331.1993.
3
Transcription factors are required for the meiotic recombination hotspot at the HIS4 locus in Saccharomyces cerevisiae.转录因子对于酿酒酵母中HIS4基因座的减数分裂重组热点是必需的。
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6621-5. doi: 10.1073/pnas.90.14.6621.
4
Meiosis-induced double-strand break sites determined by yeast chromatin structure.由酵母染色质结构决定的减数分裂诱导双链断裂位点
Science. 1994 Jan 28;263(5146):515-8. doi: 10.1126/science.8290959.
5
Repetitive DNA sequences located in the terminal portion of the Caenorhabditis elegans chromosomes.位于秀丽隐杆线虫染色体末端部分的重复DNA序列。
Nucleic Acids Res. 1993 Mar 11;21(5):1133-9. doi: 10.1093/nar/21.5.1133.
6
Meiosis-specific double-strand DNA breaks at the HIS4 recombination hot spot in the yeast Saccharomyces cerevisiae: control in cis and trans.酿酒酵母中HIS4重组热点处的减数分裂特异性双链DNA断裂：顺式和反式调控。
Mol Cell Biol. 1995 Mar;15(3):1679-88. doi: 10.1128/MCB.15.3.1679.
7
Sequence non-specific double-strand breaks and interhomolog interactions prior to double-strand break formation at a meiotic recombination hot spot in yeast.酵母减数分裂重组热点处双链断裂形成之前的序列非特异性双链断裂及同源染色体间相互作用。
EMBO J. 1995 Oct 16;14(20):5115-28. doi: 10.1002/j.1460-2075.1995.tb00194.x.
8
The location and structure of double-strand DNA breaks induced during yeast meiosis: evidence for a covalently linked DNA-protein intermediate.酵母减数分裂过程中诱导产生的双链DNA断裂的位置和结构：共价连接的DNA-蛋白质中间体的证据。
EMBO J. 1995 Sep 15;14(18):4599-608. doi: 10.1002/j.1460-2075.1995.tb00139.x.
9
The nucleotide mapping of DNA double-strand breaks at the CYS3 initiation site of meiotic recombination in Saccharomyces cerevisiae.酿酒酵母减数分裂重组CYS3起始位点处DNA双链断裂的核苷酸定位
EMBO J. 1995 Sep 15;14(18):4589-98. doi: 10.1002/j.1460-2075.1995.tb00138.x.
10
Covalent protein-DNA complexes at the 5' strand termini of meiosis-specific double-strand breaks in yeast.酵母减数分裂特异性双链断裂5'链末端的共价蛋白质-DNA复合物。
Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):11274-8. doi: 10.1073/pnas.92.24.11274.
Zotero 插件