平肠球菌ATCC 9790两个rrn操纵子中基因间间隔区的特征分析
Characterization of intergenic spacers in two rrn operons of Enterococcus hirae ATCC 9790.
作者信息
Sechi L A, Daneo-Moore L
机构信息
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
出版信息
J Bacteriol. 1993 May;175(10):3213-9. doi: 10.1128/jb.175.10.3213-3219.1993.
Two DNA restriction enzyme fragments coding for the 3' termini of 16S rRNA, the 5' termini of 23S rRNA, and the intergenic spaces between them in Enterococcus hirae ATCC 9790 were cloned and sequenced. The intergenic space of one of these genes contains a tRNA(Ala) sequence, whereas the other does not. Nevertheless, the intergenic spaces contain several regions that exhibit high levels of sequence homology and are capable of forming structures with similar base pairs. An analysis of Southern blots of chromosomal DNA cut with one and two restriction enzymes indicated that E. hirae has a total of six rrn operons.
对平肠球菌ATCC 9790中编码16S rRNA 3'末端、23S rRNA 5'末端及其间基因间隔区的两个DNA限制性酶切片段进行了克隆和测序。其中一个基因的基因间隔区含有一个tRNA（丙氨酸）序列，而另一个则没有。然而，这些基因间隔区包含几个具有高度序列同源性且能够形成具有相似碱基对结构的区域。用一种和两种限制性酶切割染色体DNA的Southern杂交分析表明，平肠球菌共有六个rrn操纵子。
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