Finn P J, Gibson N J, Fallon R, Hamilton A, Brown T
Department of Chemistry, University of Southampton, Highfield, UK.
Nucleic Acids Res. 1996 Sep 1;24(17):3357-63. doi: 10.1093/nar/24.17.3357.
Adenine, thymine and cytosine PNA monomers have been prepared using 3-amino-1,2-propanediol as a starting material. The benzoyl group was used to protect the exocyclic amines of the heterocyclic bases of A and C PNA monomers and the backbone primary amine was protected with the monomethoxytrityl group. The thymine and cytosine PNA monomers were used in conjunction with standard DNA synthesis monomers to produce chimeric PNA DNA (PDC) oligomers. Ultraviolet melting studies confirmed that these oligomers form stable hybrids with complementary DNA strands and that mismatches in the DNA but more so in the PNA sections lead to duplex destabilisation.
已使用3-氨基-1,2-丙二醇作为起始原料制备了腺嘌呤、胸腺嘧啶和胞嘧啶PNA单体。苯甲酰基用于保护A和C PNA单体杂环碱基的环外胺，主链伯胺用单甲氧基三苯甲基保护。胸腺嘧啶和胞嘧啶PNA单体与标准DNA合成单体结合使用，以产生嵌合PNA-DNA（PDC）寡聚物。紫外熔解研究证实，这些寡聚物与互补DNA链形成稳定的杂交体，并且DNA中的错配，尤其是PNA部分中的错配会导致双链体不稳定。
