Otto P, Elschner M, Reinhold P, Köhler H, Streckert H J, Philippou S, Werchau H, Morgenroth K
Federal Institute for Health Protection of Consumers and Veterinary Medicine, Jena, Germany.
Comp Immunol Microbiol Infect Dis. 1996 Feb;19(2):85-97. doi: 10.1016/0147-9571(95)00030-5.
Five conventionally kept calves aged between 17 and 24 days were experimentally infected with bovine respiratory syncytial virus (BRSV) by aerosol in order to mimic the natural infection route. The calves were killed and autopsies performed 7 days after the first virus challenge. The BRSV isolate used induced tracheitis, bronchitis and atelectasis in infected calves. The only virus which could be isolated from the lungs of the calves was BRSV. In addition, Mycoplasma bovirhinis was isolated from the lungs or/and trachea of two calves. The clinical and histopathological findings, as well as the detection of BRSV antigens by immunofluorescence in the epithelial cells of lung and trachea, and the reisolation of the virus from bronchoalveolar lavage fluids of all inoculated calves, provided confirmation of successful infection with BRSV.
为模拟自然感染途径，对5头17至24日龄常规饲养的犊牛进行气溶胶感染牛呼吸道合胞病毒（BRSV）实验。首次病毒攻击7天后，处死犊牛并进行解剖。所使用的BRSV分离株在感染的犊牛中引发了气管炎、支气管炎和肺不张。犊牛肺部唯一可分离出的病毒是BRSV。此外，从两头犊牛的肺或/和气管中分离出了牛鼻支原体。临床和组织病理学发现，以及通过免疫荧光在肺和气管上皮细胞中检测到BRSV抗原，以及从所有接种犊牛的支气管肺泡灌洗液中再次分离出病毒，均证实成功感染了BRSV。
