使用不同亚型单克隆抗体混合物通过直接免疫荧光法对甲型流感病毒、乙型流感病毒和呼吸道合胞病毒感染进行鉴别诊断。
Differential diagnoses of influenza A virus, influenza B virus, and respiratory syncytial virus infections by direct immunofluorescence using mixtures of monoclonal antibodies of different isotypes.
作者信息
Murphy P, Roberts Z M, Waner J L
机构信息
Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, USA.
出版信息
J Clin Microbiol. 1996 Jul;34(7):1798-800. doi: 10.1128/JCM.34.7.1798-1800.1996.
Abstract
Differences between isotypes of monoclonal antibodies were employed to detect influenza A and B viruses and respiratory syncytial virus by direct immunofluorescence using fluorescein isothiocyanate or Texas Red conjugates. Examination of 56 specimens for influenza A and B viruses and 112 specimens for influenza A virus and respiratory syncytial virus showed the mixed-isotype test to be comparable to the conventional procedure.
摘要
利用单克隆抗体不同同型之间的差异，通过使用异硫氰酸荧光素或德克萨斯红缀合物的直接免疫荧光法检测甲型和乙型流感病毒以及呼吸道合胞病毒。对56份检测甲型和乙型流感病毒的标本以及112份检测甲型流感病毒和呼吸道合胞病毒的标本进行检测，结果表明混合同型检测与传统方法相当。
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