通过单一聚合酶链反应检测血液培养物中的流感嗜血杆菌和肺炎链球菌DNA。
Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay.
作者信息
Hassan-King M, Baldeh I, Adegbola R, Omosigho C, Usen S O, Oparaugo A, Greenwood B M
机构信息
Medical Research Council Laboratories, Fajara, The Gambia, West Africa.
出版信息
J Clin Microbiol. 1996 Aug;34(8):2030-2. doi: 10.1128/jcm.34.8.2030-2032.1996.
Abstract
A multiplex PCR assay was developed to screen blood cultures from children in The Gambia with suspected pneumonia for the simultaneous detection of Haemophilus influenzae type b and Streptococcus pneumoniae isolates. Analysis of 295 blood cultures showed that PCR detected the organisms in all samples positive by culture in two samples infected with H. influenzae type b and four samples infected with S. pneumoniae that were culture negative, indicating that this method is sensitive for detecting these organisms in blood cultures.
摘要
开发了一种多重PCR检测方法，用于筛查冈比亚疑似肺炎儿童的血培养物，以同时检测b型流感嗜血杆菌和肺炎链球菌分离株。对295份血培养物的分析表明，PCR在2份感染b型流感嗜血杆菌且培养阴性的样本以及4份感染肺炎链球菌且培养阴性的样本中，检测到了所有培养阳性样本中的病原体，这表明该方法在血培养物中检测这些病原体具有敏感性。
相似文献
1
Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay.通过单一聚合酶链反应检测血液培养物中的流感嗜血杆菌和肺炎链球菌DNA。
J Clin Microbiol. 1996 Aug;34(8):2030-2. doi: 10.1128/jcm.34.8.2030-2032.1996.
2
3
4
Identification of Streptococcus pneumoniae and Haemophilus influenzae in culture-negative middle ear fluids from children with acute otitis media by combination of multiplex PCR and multi-locus sequencing typing.通过多重聚合酶链反应（PCR）和多位点测序分型相结合的方法，在急性中耳炎患儿培养阴性的中耳积液中鉴定肺炎链球菌和流感嗜血杆菌。
Int J Pediatr Otorhinolaryngol. 2011 Feb;75(2):239-44. doi: 10.1016/j.ijporl.2010.11.008. Epub 2010 Dec 3.
5
Multiplex polymerase chain reaction detection of Streptococcus pneumoniae and Haemophilus influenzae and their antibiotic resistance in patients with community-acquired pneumonia from southwest Iran.伊朗西南部社区获得性肺炎患者中肺炎链球菌和流感嗜血杆菌的多重聚合酶链反应检测及其抗生素耐药性。
BMC Microbiol. 2021 Dec 14;21(1):343. doi: 10.1186/s12866-021-02408-7.
6
Bacteria from bronchoalveolar lavage fluid from children with suspected chronic lower respiratory tract infection: results from a multi-center, cross-sectional study in Spain.儿童疑似慢性下呼吸道感染支气管肺泡灌洗液中的细菌：来自西班牙多中心、横断面研究的结果。
Eur J Pediatr. 2018 Feb;177(2):181-192. doi: 10.1007/s00431-017-3044-3. Epub 2017 Dec 29.
7
A multiplex PCR-based reverse line blot hybridization (mPCR/RLB) assay for detection of bacterial respiratory pathogens in children with pneumonia.一种基于多重聚合酶链反应的反向线印迹杂交（mPCR/RLB）检测方法用于检测肺炎患儿的细菌性呼吸道病原体。
Pediatr Pulmonol. 2008 Feb;43(2):150-9. doi: 10.1002/ppul.20749.
8
A polymerase chain reaction for the diagnosis of Haemophilus influenzae type b disease in children and its evaluation during a vaccine trial.用于诊断儿童b型流感嗜血杆菌疾病的聚合酶链反应及其在疫苗试验中的评估。
Pediatr Infect Dis J. 1998 Apr;17(4):309-12. doi: 10.1097/00006454-199804000-00008.
9
Haemophilus influenzae type-b and non-b-type invasive diseases in urban children (<5years) of Bangladesh: implications for therapy and vaccination.孟加拉国城市地区5岁以下儿童中b型和非b型流感嗜血杆菌侵袭性疾病：对治疗和疫苗接种的启示
J Infect. 2008 Mar;56(3):191-6. doi: 10.1016/j.jinf.2007.12.008.
10
Etiology of severe childhood pneumonia in the Gambia, West Africa, determined by conventional and molecular microbiological analyses of lung and pleural aspirate samples.通过对肺和胸腔穿刺抽吸样本进行传统及分子微生物学分析确定西非冈比亚儿童重症肺炎的病因
Clin Infect Dis. 2014 Sep 1;59(5):682-5. doi: 10.1093/cid/ciu384. Epub 2014 May 27.
引用本文的文献
1
Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease.莫桑比克致病性小儿细菌性脑膜炎的发病率：多重实时聚合酶链反应在估计疾病负担中的关键作用
PLoS One. 2015 Sep 22;10(9):e0138249. doi: 10.1371/journal.pone.0138249. eCollection 2015.
2
PCR identification of bacteria in blood culture does not fit the daily workflow of a routine microbiology laboratory.血培养中细菌的 PCR 鉴定与常规微生物实验室的日常工作流程不匹配。
J Clin Microbiol. 2012 Mar;50(3):1031-3. doi: 10.1128/JCM.01271-11. Epub 2011 Dec 14.
3
PCR using blood for diagnosis of invasive pneumococcal disease: systematic review and meta-analysis.采用血液进行 PCR 检测以诊断侵袭性肺炎球菌病：系统评价和荟萃分析。
J Clin Microbiol. 2010 Feb;48(2):489-96. doi: 10.1128/JCM.01636-09. Epub 2009 Dec 9.
4
Detection of Haemophilus influenzae type b by real-time PCR.通过实时聚合酶链反应检测b型流感嗜血杆菌
J Clin Microbiol. 2004 Aug;42(8):3813-5. doi: 10.1128/JCM.42.8.3813-3815.2004.
5
The role of DNA amplification technology in the diagnosis of infectious diseases.DNA扩增技术在传染病诊断中的作用。
CMAJ. 2000 Aug 8;163(3):301-9. doi: 10.1016/s1381-1169(00)00220-x.
6
Rapid identification of nine microorganisms causing acute respiratory tract infections by single-tube multiplex reverse transcription-PCR: feasibility study.单管多重逆转录聚合酶链反应快速鉴定九种引起急性呼吸道感染的微生物：可行性研究
J Clin Microbiol. 1999 Jan;37(1):1-7. doi: 10.1128/JCM.37.1.1-7.1999.
7
PCR-Enzyme immunoassay for detection of Streptococcus pneumoniae DNA in cerebrospinal fluid samples from patients with culture-negative meningitis.用于检测培养阴性脑膜炎患者脑脊液样本中肺炎链球菌DNA的聚合酶链反应-酶免疫测定法
J Clin Microbiol. 1998 Dec;36(12):3605-8. doi: 10.1128/JCM.36.12.3605-3608.1998.
本文引用的文献
1
Importance of enteric bacteria as a cause of pneumonia, meningitis and septicemia among children in a rural community in The Gambia, West Africa.
Pediatr Infect Dis J. 1994 Feb;13(2):122-8. doi: 10.1097/00006454-199402000-00009.
2
Detection of Streptococcus pneumoniae DNA in blood cultures by PCR.通过聚合酶链反应检测血培养中的肺炎链球菌DNA。
J Clin Microbiol. 1994 Jul;32(7):1721-4. doi: 10.1128/jcm.32.7.1721-1724.1994.
3
Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and streptococci using a seminested PCR strategy.采用半巢式聚合酶链反应（PCR）策略同时检测脑膜炎奈瑟菌、流感嗜血杆菌和链球菌的方法检测脑脊液中的细菌DNA。
J Clin Microbiol. 1994 Nov;32(11):2738-44. doi: 10.1128/jcm.32.11.2738-2744.1994.
4
PCR for capsular typing of Haemophilus influenzae.用于流感嗜血杆菌荚膜分型的聚合酶链反应
J Clin Microbiol. 1994 Oct;32(10):2382-6. doi: 10.1128/jcm.32.10.2382-2386.1994.
5
The etiology of lobar pneumonia in the Gambia.冈比亚大叶性肺炎的病因
Bull World Health Organ. 1986;64(4):553-8.
6
Etiology of acute lower respiratory tract infections in Gambian children: II. Acute lower respiratory tract infection in children ages one to nine years presenting at the hospital.冈比亚儿童急性下呼吸道感染的病因：II. 到医院就诊的1至9岁儿童的急性下呼吸道感染
Pediatr Infect Dis J. 1991 Jan;10(1):42-7. doi: 10.1097/00006454-199101000-00009.
7
Etiology of acute lower respiratory tract infections in Gambian children: I. Acute lower respiratory tract infections in infants presenting at the hospital.冈比亚儿童急性下呼吸道感染的病因：I. 到医院就诊的婴儿的急性下呼吸道感染
Pediatr Infect Dis J. 1991 Jan;10(1):33-41. doi: 10.1097/00006454-199101000-00008.
8
Diagnosis of acute bacterial pneumonia in Nigerian children. Value of needle aspiration of lung of countercurrent immunoelectrophoresis.尼日利亚儿童急性细菌性肺炎的诊断。肺穿刺及对流免疫电泳的价值。
Arch Dis Child. 1977 Dec;52(12):925-31. doi: 10.1136/adc.52.12.925.
Zotero 插件