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J Clin Microbiol. 1996 Aug;34(8):2030-2. doi: 10.1128/jcm.34.8.2030-2032.1996.
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本文引用的文献

1
Importance of enteric bacteria as a cause of pneumonia, meningitis and septicemia among children in a rural community in The Gambia, West Africa.
Pediatr Infect Dis J. 1994 Feb;13(2):122-8. doi: 10.1097/00006454-199402000-00009.
2
Detection of Streptococcus pneumoniae DNA in blood cultures by PCR.通过聚合酶链反应检测血培养中的肺炎链球菌DNA。
J Clin Microbiol. 1994 Jul;32(7):1721-4. doi: 10.1128/jcm.32.7.1721-1724.1994.
3
Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and streptococci using a seminested PCR strategy.采用半巢式聚合酶链反应（PCR）策略同时检测脑膜炎奈瑟菌、流感嗜血杆菌和链球菌的方法检测脑脊液中的细菌DNA。
J Clin Microbiol. 1994 Nov;32(11):2738-44. doi: 10.1128/jcm.32.11.2738-2744.1994.
4
PCR for capsular typing of Haemophilus influenzae.用于流感嗜血杆菌荚膜分型的聚合酶链反应
J Clin Microbiol. 1994 Oct;32(10):2382-6. doi: 10.1128/jcm.32.10.2382-2386.1994.
5
The etiology of lobar pneumonia in the Gambia.冈比亚大叶性肺炎的病因
Bull World Health Organ. 1986;64(4):553-8.
6
Etiology of acute lower respiratory tract infections in Gambian children: II. Acute lower respiratory tract infection in children ages one to nine years presenting at the hospital.冈比亚儿童急性下呼吸道感染的病因：II. 到医院就诊的1至9岁儿童的急性下呼吸道感染
Pediatr Infect Dis J. 1991 Jan;10(1):42-7. doi: 10.1097/00006454-199101000-00009.
7
Etiology of acute lower respiratory tract infections in Gambian children: I. Acute lower respiratory tract infections in infants presenting at the hospital.冈比亚儿童急性下呼吸道感染的病因：I. 到医院就诊的婴儿的急性下呼吸道感染
Pediatr Infect Dis J. 1991 Jan;10(1):33-41. doi: 10.1097/00006454-199101000-00008.
8
Diagnosis of acute bacterial pneumonia in Nigerian children. Value of needle aspiration of lung of countercurrent immunoelectrophoresis.尼日利亚儿童急性细菌性肺炎的诊断。肺穿刺及对流免疫电泳的价值。
Arch Dis Child. 1977 Dec;52(12):925-31. doi: 10.1136/adc.52.12.925.

通过单一聚合酶链反应检测血液培养物中的流感嗜血杆菌和肺炎链球菌DNA。

Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay.

作者信息

Hassan-King M, Baldeh I, Adegbola R, Omosigho C, Usen S O, Oparaugo A, Greenwood B M

机构信息

Medical Research Council Laboratories, Fajara, The Gambia, West Africa.

出版信息

J Clin Microbiol. 1996 Aug;34(8):2030-2. doi: 10.1128/jcm.34.8.2030-2032.1996.

DOI:10.1128/jcm.34.8.2030-2032.1996
PMID:8818907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229179/
Abstract

A multiplex PCR assay was developed to screen blood cultures from children in The Gambia with suspected pneumonia for the simultaneous detection of Haemophilus influenzae type b and Streptococcus pneumoniae isolates. Analysis of 295 blood cultures showed that PCR detected the organisms in all samples positive by culture in two samples infected with H. influenzae type b and four samples infected with S. pneumoniae that were culture negative, indicating that this method is sensitive for detecting these organisms in blood cultures.

摘要

开发了一种多重PCR检测方法，用于筛查冈比亚疑似肺炎儿童的血培养物，以同时检测b型流感嗜血杆菌和肺炎链球菌分离株。对295份血培养物的分析表明，PCR在2份感染b型流感嗜血杆菌且培养阴性的样本以及4份感染肺炎链球菌且培养阴性的样本中，检测到了所有培养阳性样本中的病原体，这表明该方法在血培养物中检测这些病原体具有敏感性。